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Automated proteomics workflows for consistent library generation and label-free protein quantitation in 96-well format and across different samples types

ABSTRACT: Automated workflows for high-throughput library generation and biomarker detection using data-independent acquisition

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Heart

DISEASE(S): Inferior Myocardial Infarction

SUBMITTER: Selvam Paramasivan

LAB HEAD: Pawel Sadowski

PROVIDER: PXD038413 | Pride | 2023-04-22

REPOSITORIES: Pride

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			Action	DRS
	20201022D_SP1189_IMI_32_SWATH_R1.wiff	Wiff
	20201022D_SP1189_IMI_32_SWATH_R1.wiff.scan	Wiff
	20201022D_SP1190_IMI_32_SWATH_R2.wiff	Wiff
	20201022D_SP1190_IMI_32_SWATH_R2.wiff.scan	Wiff
	20201022D_SP1191_IMI_32_SWATH_R3.wiff	Wiff

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Publications

Automated Proteomics Workflows for High-Throughput Library Generation and Biomarker Detection Using Data-Independent Acquisition.

Paramasivan Selvam S Morrison Janna L JL Lock Mitchell C MC Darby Jack R T JRT Barrero Roberto A RA Mills Paul C PC Sadowski Pawel P

Journal of proteome research 20230523 6

Sequential window acquisition of all theoretical mass spectra-mass spectrometry underpinned by advanced bioinformatics offers a framework for comprehensive analysis of proteomes and the discovery of robust biomarkers. However, the lack of a generic sample preparation platform to tackle the heterogeneity of material collected from different sources may be a limiting factor to the broad application of this technique. We have developed universal and fully automated workflows using a robotic sample ...[more]

PMID: 37219895

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