Dataset Information

Proteomics data reveals Wnt signaling involvement in the hippocampal-dependent behavioral impairment in THRSP-overexpressing ADHD mouse model

ABSTRACT: Despite the predominance and high heritability of Attention-Deficit/Hyperactivity Disorder (ADHD), its etiology remains elusive. Preclinical and clinical evidence partly points to impaired limbic system function, particularly that of the hippocampus (HPC). Children diagnosed with ADHD often struggle with deficits in executive function, temporal processing, and visuospatial memory, the defining hallmarks of the predominantly inattentive presentation (ADHD-PI), thought to be subserved by the HPC brain region. However, the specific genes/proteins involved and how they shape the hippocampal makeup to influence ADHD behavior are poorly understood. As an exploratory tool, hippocampal tissues from a mouse model overexpressing thyroid hormone-responsive protein (THRSP), with defining characteristics of ADHD-PI presentation, were utilized for proteomic analyses. Results revealed differential expressions of proteins involved in Wnt signaling. Compared to THRSP knockout (KO), THRSP OE mice have impaired attention and memory concomitant to dysregulated Wnt signaling affecting hippocampal cell proliferation (BrdU) and neurogenic markers expressions (i.e., NEU-N, GFAP), markers of neural stem cell (NSC) activity. Also, exposure to a combination of enriched environment and treadmill exercise improves behavioral deficits in THRSP OE mice and improves Wnt signaling and NSC activity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Jiyeon Kim

LAB HEAD: Eugene C. Yi

PROVIDER: PXD038525 | Pride | 2023-01-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210831_ADHD_HPC_KO_F01_01.mgf	Mgf
	20210831_ADHD_HPC_KO_F01_01.raw	Raw
	20210831_ADHD_HPC_KO_F02_01.raw	Raw
	20210831_ADHD_HPC_KO_F03_01.raw	Raw
	20210831_ADHD_HPC_KO_F04_01.raw	Raw

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Publications

Hippocampal dentate gyri proteomics reveals Wnt signaling involvement in the behavioral impairment in the THRSP-overexpressing ADHD mouse model.

Custodio Raly James Perez RJP Kim Hee Jin HJ Kim Jiyeon J Ortiz Darlene Mae DM Kim Mikyung M Buctot Danilo D Sayson Leandro Val LV Lee Hyun Jun HJ Kim Bung-Nyun BN Yi Eugene C EC Cheong Jae Hoon JH

Communications biology 20230116 1

Children with attention-deficit/hyperactivity disorder (ADHD) often struggle with impaired executive function, temporal processing, and visuospatial memory, hallmarks of the predominantly inattentive presentation (ADHD-PI), subserved by the hippocampus. However, the specific genes/proteins involved and how they shape hippocampal structures to influence ADHD behavior remain poorly understood. As an exploratory tool, hippocampal dentate gyri tissues from thyroid hormone-responsive protein overexpr ...[more]

PMID: 36646879

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