Proteomics

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Retinal proteome analysis reveals region-specific change in the rabbit myopia model


ABSTRACT: Uncovering region-specific change in myopic retina can provide the clue for explaining the pathogenesis of myopia progression. After imposing form deprivation myopia (FDM) on the right eye of 6-week-old rabbits, we investigated the proteome profile of each retinal region (central, mid periphery, and far periphery retina) using high-resolution, accurate mass (HRAM) mass spectrometry. Protein expression was analyzed with gene ontology and network analysis compared to the control, the left eyes. Among total 2065 proteins detected from whole retinal samples, 249 differentially-expressed proteins (DEPs) were identified: 164 DEPs in far periphery; 39 DEPs in mid periphery; 83 DEPs in central retina. In network analysis, far periphery retina showed the most significant connectivity between DEPs. Regulation of coagulation was the most significant biological process in up-regulated DEPs in far periphery retina. Proteasome was the most significant KEGG pathway in down-regulated DEPs in central retina. Antithrombin-III, fibrinogen gamma chain, and fibrinogen beta chain were identified as biomarkers for myopia progression, which were up-regulated in far periphery retina. Proteomic analysis in this study suggested that the oxidative stress can be the main pathogenesis of myopia progression and the far periphery retina played a role as the key responder.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Retinal Cell, Retina

DISEASE(S): Myopia

SUBMITTER: Chae Eun Moon  

LAB HEAD: Yuri Seo

PROVIDER: PXD038552 | Pride | 2023-03-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Ctrl_central.raw Raw
Ctrl_central_210707233021.raw Raw
Ctrl_central_210708013855.raw Raw
Ctrl_far.raw Raw
Ctrl_far_210708163854.raw Raw
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Publications

Retinal Proteome Analysis Reveals a Region-Specific Change in the Rabbit Myopia Model.

Moon Chae-Eun CE   Ji Yong Woo YW   Lee Jun-Ki JK   Han Kyusun K   Kim Hyunjin H   Byeon Seok Ho SH   Han Suenghan S   Han Jinu J   Seo Yuri Y  

International journal of molecular sciences 20230109 2


Uncovering region-specific changes in the myopic retina can provide clues to the pathogenesis of myopia progression. After imposing form deprivation myopia in the right eye of 6-week-old rabbits, we investigated the proteome profile of each retinal region (central, mid-periphery, and far-periphery retina), using accurate high-resolution mass spectrometry. Protein expression was analyzed using gene ontology and network analysis compared with that of the control, the left eyes. Among 2065 proteins  ...[more]

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