Dataset Information

Posttranslation modification of NF-kB inducing kinase

ABSTRACT: 2 This project is to identify the potential neddylation sites in human NF-kB inducing kinase (A.k.a. Map3K14). NIK-HA or NIK-HA in combination with FLAG-NEDD8 were co-expressed in HEK293T cells. NIK-HA were pulled down via denaturing immunoprecipitation protocol, subject to SDS-PAGE, in-gel trypsin digestion, and Nano LC-MS/MS Analysis. The potential peptides containing K-GG sites were identified.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte

DISEASE(S): Liver Disease

SUBMITTER: Weiqin Chen

LAB HEAD: Weiqin Chen

PROVIDER: PXD038653 | Pride | 2023-03-10

REPOSITORIES: Pride

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Publications

Hepatic neddylation deficiency triggers fatal liver injury via inducing NF-κB-inducing kinase in mice.

Xu Cheng C Zhou Hongyi H Jin Yulan Y Sahay Khushboo K Robicsek Anna A Liu Yisong Y Dong Kunzhe K Zhou Jiliang J Barrett Amanda A Su Huabo H Chen Weiqin W

Nature communications 20221216 1

The conjugation of neural precursor cell expressed, developmentally downregulated 8 (NEDD8) to target proteins, termed neddylation, participates in many cellular processes and is aberrant in various pathological diseases. Its relevance to liver function and failure remains poorly understood. Herein, we show dysregulated expression of NAE1, a regulatory subunit of the only NEDD8 E1 enzyme, in human acute liver failure. Embryonic- and adult-onset deletion of NAE1 in hepatocytes causes hepatocyte d ...[more]

PMID: 36526632

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			Action	DRS
	GPLCLKIASEPPPVR.pdf	Pdf
	LKPVDYEYR.pdf	Pdf
	NIK-NIK+NEDD8-database searching result.xlsx	Xlsx
	NIK.raw	Raw
	NIKN8.raw	Raw