Dataset Information

Upregulation of filaggrin 2 in scleritis using tissue mass spectrometry

ABSTRACT: Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. Using a mass spectrometry approach, we investigated healthy sclera and sclera affected by non-infectious scleritis for differentially expressed proteins. Therefore, we collected scleral samples of enucleated eyes due to severe non-infectious scleritis (n=3) and control scleral tissues (n=5), all exenterated eyes for eyelid carcinomas without scleral invasion. Nano LC-MS mass spectrometry identified 629 proteins within the healthy and diseased scleral tissues, of which collagen type I was the most abundantly expressed protein. Collagen type II-XII was also present. Filaggrin-2, a protein that plays a crucial role in epidermal barrier function, was upregulated in all scleritis cases. In addition, other epithelial-associated proteins were upregulated (such as Keratin 33b, 34, and 85, Epiplakin, transglutaminase-3, Galectin 7, and Caspase-14) in scleritis. Further, upregulated proteins involved in regulation of the cytoskeleton (Vinculin and Myosin 9) and housekeeping proteins were found (elongation factor-2 and cytoplasmic dynein 1). We selected two proteins for validation with immunohistochemistry: Fillagrin-2 and myosin 9. Upregulation of both proteins was confirmed, the latter protein showing co-localization with the endothelial cell marker ETC-related gene (ERG), indicating neovascularization in scleral tissue affected in scleritis. Further research, preferably with multiple scleritis cases, is needed to validate our findings, including identification of the specific role of Filaggrin-2 and angiogenesis in the pathogenesis of scleritis.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Coskun Guzel

LAB HEAD: Dr. T.M. Luider

PROVIDER: PXD038727 | Pride | 2023-05-10

REPOSITORIES: Pride

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			Action	DRS
	210925OEc1_CG-2091-WTL-1233-03a-DDA_002.mgf	Mgf
	210925OEc1_CG-2091-WTL-1233-03a-DDA_002.raw	Raw
	210925OEc1_CG-2091-WTL-1233-03a-DDA_002__F155816_.mzid.gz	Mzid
	210925OEc1_CG-2091-WTL-1233-03a-DDA_002__F155816_.mzid_210925OEc1_CG-2091-WTL-1233-03a-DDA_002__F155816_.MGF	Mzid
	210925OEc1_CG-2091-WTL-1233_03b-DDA_004.mgf	Mgf

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Publications

Scleral Proteome in Noninfectious Scleritis Unravels Upregulation of Filaggrin-2 and Signs of Neovascularization.

Vergouwen Daphne P C DPC Ten Berge Josianne C JC Guzel Coskun C van den Bosch Thierry P P TPP Verdijk Robert M RM Rothova Aniki A Luider Theo M TM Schreurs Marco W J MWJ

Investigative ophthalmology & visual science 20230301 3

<h4>Purpose</h4>Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. We investigated healthy sclera as well as sclera affected by noninfectious scleritis for differentially expressed proteins using a mass spectrometry approach.<h4>Methods</h4>We collected scleral samples of enucleated eyes due to severe noninfectious scleritis (n = 3), and control scleral tissues (n = 5), all exenterated eyes for eyelid carcinomas (n = 4), or choroidal melanoma (n = 1) without scleral in ...[more]

PMID: 36930145

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