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Caulobacter crescentus flagellins glycopeptides analyses

ABSTRACT: Caulobacter crescentus is an alphaproteobacterium that divides assymetrically. Each cell cycle results in the production of a motile flagellated cell and a sessile cell called the swamer cell and the stalked cell, respectively. The flagellar filament is composed of thousands polymerized flagellins. We showed that glycosylation of flagellins is required for the assembly of the flagellum. This glycosylation is performed by soluble FlmG glycosyltransferases that transfer nonulosonic acids (pseudaminic acid or legionaminic acid) directly to the flagellins. Such glycosylation system is also present in a close relative of Caulobacter crescentus, Brevundimonas subvibrioides. The project is to identify the site of glycosylation and the potential sugar added on this site.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Caulobacter Crescentus (strain Na1000 / Cb15n) Brevundimonas Subvibrioides Atcc 15264

SUBMITTER: Nicolas Kint

LAB HEAD: Patrick Viollier

PROVIDER: PXD038730 | Pride | 2023-01-13

REPOSITORIES: Pride

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			Action	DRS
	20220707_002_S399294_FljK-WT_A.raw	Raw
	20220707_002_S399294_FljK-WT_A.raw_20220711_Byonic_T220_WC_130_600.mgf	Mgf
	20220707_002_S399294_FljK-WT_A.raw_20220711_Byonic_T220_WC_130_600.mzid.gz	Mzid
	20220707_004_S399295_FljK-pseI_A.raw	Raw
	20220707_004_S399295_FljK-pseI_A.raw_20220711_Byonic_T220_WC_130_600.mgf	Mgf

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