Proteomics

Dataset Information

0

Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium


ABSTRACT: The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether a similar process exists in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of Argonaute proteins of the Piwi-clade involved in various small RNA pathways, many of which have not yet been investigated. In this work, we investigated the function of a Piwi protein, Ptiwi08, whose expression is limited to a narrow time window during development, concomitant with the start of zygotic transcription. We show that Ptiwi08 acts in an endo-siRNA pathway involved in the clearance of a set of mRNAs at the post-transcriptional level. Based on their features, we hypothesized that these mRNAs may be untranslated. In this dataset, we used mass spectrometry to determine whether any protein products of these mRNAs can be found.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Paramecium Tetraurelia

SUBMITTER: Manfred Heller  

LAB HEAD: Mariusz Nowacki

PROVIDER: PXD038984 | Pride | 2023-05-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20210715_EV_TS_i01.raw Raw
20210715_EV_TS_i02.raw Raw
20210715_EV_TS_i03.raw Raw
20210715_EV_TS_i04.raw Raw
20210715_EV_TS_i05.raw Raw
Items per page:
1 - 5 of 23
altmetric image

Publications

Developmental mRNA clearance by PIWI-bound endo-siRNAs in Paramecium.

Solberg Therese T   Mason Victor V   Wang Chundi C   Nowacki Mariusz M  

Cell reports 20230303 3


The clearance of untranslated mRNAs by Argonaute proteins is essential for embryonic development in metazoans. However, it is currently unknown whether similar processes exist in unicellular eukaryotes. The ciliate Paramecium tetraurelia harbors a vast array of PIWI-clade Argonautes involved in various small RNA (sRNA) pathways, many of which have not yet been investigated. Here, we investigate the function of a PIWI protein, Ptiwi08, whose expression is limited to a narrow time window during de  ...[more]

Similar Datasets

2022-10-17 | PXD035694 | Pride
2022-08-25 | PXD033418 | Pride
2021-05-07 | PXD022036 | Pride
2019-03-25 | PXD012881 | Pride
2022-05-30 | PXD031677 | Pride
2021-07-06 | PXD025930 | Pride
2023-06-12 | PXD041156 | Pride
2023-03-08 | PXD034848 | Pride
2024-09-27 | PXD054716 | Pride
2023-07-11 | PXD035270 | Pride