Dataset Information

TSN-interacting proteins in macrophages

ABSTRACT: TSN, a small molecular compound, was covalently attached to agarose beads via ketone or OH group. Macrophages-derived cell lysates were incubated with these TSN-conjugated beads and mass spectrometry-based proteomic analysis was performed to identify potential TSN interacting proteins

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

SUBMITTER: Fan Yang

LAB HEAD: Yi Fan

PROVIDER: PXD039067 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Experiment_FY1584_1_from_q210806_FY1584_01.mzid.gz	Mzid
	Experiment_FY1584_1_from_q210806_FY1584_01.mzid_Experiment_FY1584_1_from_q210806_FY1584_01.MGF	Mzid
	Experiment_FY1584_2_from_q210806_FY1584_02.mzid.gz	Mzid
	Experiment_FY1584_2_from_q210806_FY1584_02.mzid_Experiment_FY1584_2_from_q210806_FY1584_02.MGF	Mzid
	Experiment_FY1584_3_from_q210806_FY1584_03.mzid.gz	Mzid

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Publications

Small-molecule toosendanin reverses macrophage-mediated immunosuppression to overcome glioblastoma resistance to immunotherapy.

Yang Fan F Zhang Duo D Jiang Haowen H Ye Jiangbin J Zhang Lin L Bagley Stephen J SJ Winkler Jeffery J Gong Yanqing Y Fan Yi Y

Science translational medicine 20230215 683

T cell-based immunotherapy holds promise for treating solid tumors, but its therapeutic efficacy is limited by intratumoral immune suppression. This immune suppressive tumor microenvironment is largely driven by tumor-associated myeloid cells, including macrophages. Here, we report that toosendanin (TSN), a small-molecule compound, reprograms macrophages to enforce antitumor immunity in glioblastoma (GBM) in mouse models. Our functional screen of genetically probed macrophages with a chemical li ...[more]

PMID: 36791206

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