Dataset Information

Proteomes of acrosomes and acrosome-reacted sperm of the house mouse

ABSTRACT: Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS2) proteomics, we provide proteomes of the acrosomes and acrosome-reacted sperm isolated from cauda epididymis of free-living house mice Mus musculus musculus. Among all proteins, 334 proteins were significantly enriched in the acrosome thus representing 27.3% of the whole proteome of the intact sperm. Importantly, we have detected a total of nine calycins while eight of them belong to the lipocalin protein family. Column names in resulting tables are tagged as S1-S10 (supernatants) and represent acrosomal content (A1-A10 in Table S1) while samples P1-P10 are pellets and represent arcosome reacted sperm (S1-S10 in Table S1).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epididymis

SUBMITTER: Pavel Stopka

LAB HEAD: Pavel Stopka

PROVIDER: PXD039128 | Pride | 2023-02-27

REPOSITORIES: Pride

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Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality.

Otčenášková Tereza T Macíčková Eliška E Vondráková Jana J Frolíková Michaela M Komrskova Katerina K Stopková Romana R Stopka Pavel P

European journal of cell biology 20230215 2

The acrosome located within the mammalian sperm head is essential for successful fertilization, as it enables the sperm to penetrate the extracellular layers of the oocyte and fuse with oolemma. However, the mammalian acrosomal vesicle is no longer considered to contain only hydrolytic enzymes. Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS/MS) proteomics, we identified a total of 885 proteins in the acrosome isolated from spermatozoa obtained from cauda epidi ...[more]

PMID: 36805822

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			Action	DRS
	20200121_Stopka.rar	Other
	510-10P.raw	Raw
	510-10S.raw	Raw
	510-1P.raw	Raw
	510-1S.raw	Raw