Proteomics

Dataset Information

0

Growth hormone supplementation reduces aneuploidy and improves the quality of oocytes by activating ERK1/2 pathway in aged mice

ABSTRACT: The decline in oocyte quality is a limiting factor of female fertility; however, strategies to maintain the oocyte quality of aged women are not available. In this study, we showed that growth hormone (GH) supplementation in vivo not only alleviated the decline in oocyte number caused by aging, but also improved the quality and developmental potential of aged oocytes. Strikingly, GH supplementation reduced aneuploidy in aged oocytes. Proteomic analysis indicated that the ERK1/2 pathway was involved in the reduction in aneuploidy rate of aged oocytes, as confirmed both in vivo and in vitro. In addition, JAK2 might be involved in the regulation of ERK1/2 by GH in aged oocytes. Collectively, our findings revealed that GH supplementation protects oocytes from aging-related aneuploidy and enhances the quality of aged oocytes, and could be used to improve the outcome of assisted reproduction in aged women.

INSTRUMENT(S): autoflex

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Oocyte

SUBMITTER: Yunyao Luo  

LAB HEAD: Yun-Yao Luo

PROVIDER: PXD039147 | Pride | 2023-07-20

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
RD175SC_G1_B6_1_17037.d.zip Other
RD175SC_G2_B7_1_17038.d.zip Other
RD175SC_G3_B8_1_17039.d.zip Other
RD175SC_G4_C1_1_17041.d.zip Other
RD175SC_G5_C2_1_17042.d.zip Other
Items per page:
1 - 5 of 12
altmetric image

Publications

Growth hormone reduces aneuploidy and improves oocytes quality by JAK2-MAPK3/1 pathway in aged mice.

Luo Yun-Yao YY   Zeng Xi X   Zhu Ling L   Li Chong C   Xie Juan J   Dong Qiang Q   Sun Qing-Yuan QY   Huang Guo-Ning GN   Li Jing-Yu JY  

Journal of translational medicine 20230629 1

<h4>Background</h4>The global delay in women's reproductive age has raised concerns about age-related infertility. The decline in oocyte quality is a limiting factor of female fertility, yet there are currently no strategies to preserve oocyte quality in aged women. Here, we investigated the effects of growth hormone (GH) supplementation on aneuploidy of aged oocytes.<h4>Methods</h4>For the in vivo experiments, the aged mice (8-month-old) were intraperitoneally injected with GH daily for 8 weeks  ...[more]

Similar Datasets

Proteomics Cordycepin Delays Postovulatory Aging of Mammalian Oocytes through Inhibition of Maternal mRNAs Degradation via DCP1A Polyadenylation Suppression
2024-01-26 | PXD042600 | Pride
Proteomics Quantitative proteomic analysis of oocytes from high-fat diet (HFD)-fed and normal diet mice
2018-05-24 | PXD006641 | Pride
Transcriptomics Microarray data of prepubertal, peripubertal, and adult oocytes, and from GV and MII oocytes matured in vivo or in vitro
2010-07-12 | E-GEOD-12432 | biostudies-arrayexpress
Proteomics Proteomic-based identification of oocyte maturation-related proteins in mouse GV oocytes
2020-09-17 | PXD014314 | Pride
Other Granulosa cell mevalonate pathway abnormalities contribute to oocyte meiotic defects and aneuploidy [HiC GGOH-treatment]
2023-02-26 | GSE212860 | GEO
Transcriptomics RNA-Seq profiling of Sirt1 deficient mouse oocytes to investigate Sirt1 role in maternal-zygotic transition
2024-07-06 | E-MTAB-13483 | biostudies-arrayexpress
Transcriptomics Nicotinamide mononucleotide supplementation reverses the declining quality of maternally aged oocytes
2023-07-01 | GSE153920 | GEO
Transcriptomics Transcriptome assay of materal mRNA degradation in Btg4-deleted oocytes
2016-03-16 | E-GEOD-71257 | biostudies-arrayexpress
Transcriptomics Mitochondrial supplementation at fertilization enhances early developmental outcome
2016-09-01 | E-GEOD-65310 | biostudies-arrayexpress
Transcriptomics Gene Expression Profiles in Immature and In vitro matured bovine Oocytes
2011-04-25 | E-GEOD-23449 | biostudies-arrayexpress