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Chromatome of AR and SUMO2/3 idenitified with RIME

ABSTRACT: We identifyied AR and SUMO2/3 chromatomes in castration-resistant prostate cancer cells (VCaP) and studied the impact on SUMOylation inhibition (SUMOi)on them utilizing small molecule inhibitor ML792. Chromatome members were identifyied with Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) in absence and presence of androgen and ML792. Mass spectrometry analyses were executed with high resolution mass spectrometry LC-MS analysis was performed by using the Evosep One liquid chromatography system coupled to a hybrid trapped ion mobility quadrupole TOF mass spectrometer (Bruker timsTOF Pro) via a CaptiveSpray nano-electrospray ion source. High-confidence chromatome members discriminated from background with SAINT analysis.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Kaisa-Mari Launonen

LAB HEAD: Jorma Palvimo

PROVIDER: PXD039179 | Pride | 2024-08-08

REPOSITORIES: Pride

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			Action	DRS
	AR_CTRL_DHT_rep1-3.d.zip	Other
	AR_CTRL_ETOHrep1-3.d.zip	Other
	AR_SUMOi_DHT_rep1-3.d.zip	Other
	AR_SUMOi_ETOH_rep1-3.d.zip	Other
	AR_start.xlsx	Xlsx

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