Dataset Information

Cellular and extracellular composition of meningeal trabeculae

ABSTRACT: The meninges are a tripartite system of membranous tissue comprised of pial, arachnoid and dural layers that cover both the brain and spinal cord. Between the arachnoid and pial layers is the subarachnoid space filled with cerebrospinal fluid (CSF). Though the meninges have long been thought to provide largely a protective function, a growing number of studies highlight this tissue to be a nest of immune activity, harboring T cells, B cells, macrophages, and a variety of other myeloid cell types during health and disease. But despite the burgeoning prospect that the meninges might play a decidedly more active role in immune and other regulatory processes than previously thought, little is known about their structural makeup. Contributing to this void, considerable technical challenges prevent sophisticated analysis of the meninges at cellular and molecular levels. In addition, removal of the brain and spinal cord from their bony encasement leads to tearing of the tenuous meninges and significant disruption of the delicate inter-membrane arrangements. Also lacking are sufficient molecular targets to identify the various meningeal structural elements, only hinted at so far by scanning electron microscopy. Accordingly, we developed a method to allow removal of brain and spinal meninges while minimizing risk of parenchymal contamination and performed shotgun proteomics on the two meningeal domains. While the vast majority of proteins at both locales overlapped, several proteins – including those of structural nature – were exclusive to brain or spinal meninges. Targeting proteins revealed in the proteomic data, the cellular and extracellular elements that provide the meninges’ structure were identified using confocal and electron microscopy, and were observed for the first time in high-resolution

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Jeremy Balsbaugh

LAB HEAD: Jeremy L. Balsbaugh

PROVIDER: PXD039294 | Pride | 2023-11-01

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20201231_jcl_ESantorella_Pachter_brain_1700ng.raw	Raw
	20201231_jcl_ESantorella_Pachter_spinalcord_1700ng.raw	Raw
	20210603_jlb_ESantorella_Pachter_SpCord.raw	Raw
	20210618_jlb_ESantorella_Pachter_brainredo_1ug.raw	Raw
	Brain_Rep1_20191009_txt.zip	Other

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Publications

Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis.

Santorella Elise E Balsbaugh Jeremy L JL Ge Shujun S Saboori Parisa P Baker David D Pachter Joel S JS

Fluids and barriers of the CNS 20231019 1

The meninges surround the brain and spinal cord, affording physical protection while also serving as a niche of neuroimmune activity. Though possessing stromal qualities, its complex cellular and extracellular makeup has yet to be elaborated, and it remains unclear whether the meninges vary along the neuroaxis. Hence, studies were carried-out to elucidate the protein composition and structural organization of brain and spinal cord meninges in normal, adult Biozzi ABH mice. First, shotgun, bottom ...[more]

PMID: 37858244

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