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IP-MS:Protein precipitated by antisense (AS) DNA oligomers crossing the junction site of circNEIL3

ABSTRACT: To identify the circNEIL3 interacting protein, we performed RNA pull-down assays after transfecting into cells with biotin-labeled sense (S) or antisense (AS) DNA oligomers crossing the junction site of circNEIL3, and the pull-down proteins were subjected to SDS-PAGE and Coomassie blue staining. ~45 kDa Band pulled down by the AS probe was specifically excised , digested and used for mass spectrometry analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Shuang Chen

LAB HEAD: Yong Peng

PROVIDER: PXD039394 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	checksum.txt	Txt
	circNEIL3_pulldown_ms.msf	Msf
	circNEIL3_pulldown_ms.raw	Raw

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Publications

circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1.

Chen Shuang S Li Ke K Guo Jiawei J Chen Hai-Ning HN Ming Yue Y Jin Yang Y Xu Fuyan F Zhang Tingting T Yang Yang Y Ye Zixia Z Liu Wenrong W Ma Hulin H Cheng Jian J Zhou Jian-Kang JK Li Zhang Z Shen Shu S Dai Lunzhi L Zhou Zong-Guang ZG Xu Heng H Peng Yong Y

Proceedings of the National Academy of Sciences of the United States of America 20230324 13

Distant metastasis is a major contributor to cancer-related mortality. However, the role of circRNAs in this process remains unclear. Herein, we profiled the circRNA expression in a cohort of 68 colorectal carcinoma (CRC) primary tumors and their paired liver metastatic lesions. By overlapping with the TGFβ-responsive circRNAs, circNEIL3 (hsa_circ_0001460) was identified as a TGFβ-repressive and metastasis-related circRNA. Functionally, circNEIL3 effectively inhibited tumor metastasis in both an ...[more]

PMID: 36961927

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