Dataset Information

Twilight length alters clock function, growth and flowering time in Arabidopsis via LHY/CCA1

ABSTRACT: In this study we developed an experimental set-up to study variation in twilight length using recently developed 7-band LED lights with fully programmable spectra and sigmoidal ramping of light intensity in controlled growth chambers. We employed this set-up to gain a comprehensive understanding of the impact of changing twilight lengths on Arabidopsis thaliana (Col-0) plants at both physiological and molecular levels. We studied the effect of a wide range of twilight lengths (0 min, 15 min, 30 min, 60 min, 90 min) on plant growth.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Rosette

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Uhrig

PROVIDER: PXD039428 | Pride | 2024-08-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20211012_LED_Twilight_TP_01_30_wt_1.raw	Raw
	20211012_LED_Twilight_TP_03_90_wt_4.raw	Raw
	20211012_LED_Twilight_TP_04_15_lhycca1_2.raw	Raw
	20211012_LED_Twilight_TP_05_0_lhycca1_1.raw	Raw
	20211012_LED_Twilight_TP_06_15_wt_1.raw	Raw

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Publications

Twilight length alters growth and flowering time in Arabidopsis via LHY/CCA1.

Mehta Devang D Scandola Sabine S Kennedy Curtis C Lummer Christina C Gallo Maria Camila Rodriguez MCR Grubb Lauren E LE Tan Maryalle M Scarpella Enrico E Uhrig R Glen RG

Science advances 20240628 26

Decades of research have uncovered how plants respond to two environmental variables that change across latitudes and over seasons: photoperiod and temperature. However, a third such variable, twilight length, has so far gone unstudied. Here, using controlled growth setups, we show that the duration of twilight affects growth and flowering time via the LHY/CCA1 clock genes in the model plant Arabidopsis. Using a series of progressively truncated no-twilight photoperiods, we also found tha ...[more]

PMID: 38941453

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Project description:Clubroot of Brassicaceae, an economically important soil borne disease, is caused by Plasmodiophora brassicae Woronin, an obligate, biotrophic protist. This disease poses a serious threat to canola and related crops in Canada and around the globe causing significant loss to seed yield. The pathogen is continuously evolving and new pathotypes are emerging, this necessitates the development of novel resistant canola cultivars to manage the disease effectively. Given that proteins play a crucial role in majority of biological processes and molecular functions, the identification of differentially abundant proteins (DAP) using proteomics information is an attractive approach to understand the plant-pathogen interactions as well as in the future development of gene specific markers for developing clubroot resistant (CR) cultivars. In this study, P. brassicae pathotype 3 (P3H) was used to challenge CR and clubroot susceptible (CS) canola lines. Root samples were collected at three distinct stages of pathogenesis, 7-, 14-, and 21-days post inoculation (DPI), protein samples were isolated, digested with trypsin and subjected to LC-MS/MS analysis. A total of 937 proteins demonstrated a significant (q < 0.05) change in abundance in at least in one of the time points when compared between control and inoculated CR-parent, CR-progeny, CS-parent, CS-progeny and 784 proteins were significantly (q < 0.05) changed in abundance in at least in one of the time points when compared between the inoculated- CR and CS root proteomes of parent and progeny across the three time points tested. Functional annotation of the differentially abundant proteins (DAPs) revealed several proteins related to calcium dependent signaling pathways in response to the pathogen. In addition, proteins related to reactive oxygen species (ROS) biochemistry, dehydrins, lignin, thaumatin, and phytohormones were identified. Among the DAPs, 74 putative proteins orthologous to CR proteins and quantitative trait loci (QTL) associated with eight CR loci in four chromosomes including chromosomes A3 and A8 were identified. In conclusion, these results have contributed to an improved understanding of the mechanisms that are involved in mediating response to P. brassicae in canola at the protein level.

Dataset Information

Twilight length alters clock function, growth and flowering time in Arabidopsis via LHY/CCA1

Dataset's files

Publications

Twilight length alters growth and flowering time in Arabidopsis via <i>LHY</i>/<i>CCA1</i>.

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