Dataset Information

Pivotal role for S-nitrosylation of DNA methyltransferase in epigenetic regulation

ABSTRACT: DNMTs catalyze the methylation of cytosine with SAM. DNA methylation affects key cellular processes by regulating gene expression, thus serving a variety of physiological and pathophysiological roles. However, the chemical mechanisms of DNA methylation by which its enzymatic activity is regulated have not been fully elucidated. We found that a critical cysteine residue in DNMT is the target of protein S-nitrosylation, leading to the attenuation of DNMT enzymatic activity and consequent aberrant regulation of gene expression during neoplastic cell proliferation. Our results demonstrate that de novo DNA methylation mediated by DNMT3 is regulated by NO, which is involved in tumor formation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Akihiro Ito

LAB HEAD: Akihiro Ito

PROVIDER: PXD039437 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	180905_QE2_123_Ito_01_SNOC_plus.mgf	Mgf
	180905_QE2_123_Ito_01_SNOC_plus.msf	Msf
	180905_QE2_123_Ito_01_SNOC_plus.raw	Raw
	F213396.dat	Other
	checksum.txt	Txt

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Publications

Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis.

Okuda Kosaku K Nakahara Kengo K Ito Akihiro A Iijima Yuta Y Nomura Ryosuke R Kumar Ashutosh A Fujikawa Kana K Adachi Kazuya K Shimada Yuki Y Fujio Satoshi S Yamamoto Reina R Takasugi Nobumasa N Onuma Kunishige K Osaki Mitsuhiko M Okada Futoshi F Ukegawa Taichi T Takeuchi Yasuo Y Yasui Norihisa N Yamashita Atsuko A Marusawa Hiroyuki H Matsushita Yosuke Y Katagiri Toyomasa T Shibata Takahiro T Uchida Koji K Niu Sheng-Yong SY Lang Nhi B NB Nakamura Tomohiro T Zhang Kam Y J KYJ Lipton Stuart A SA Uehara Takashi T

Nature communications 20230204 1

DNA methyltransferases (DNMTs) catalyze methylation at the C5 position of cytosine with S-adenosyl-L-methionine. Methylation regulates gene expression, serving a variety of physiological and pathophysiological roles. The chemical mechanisms regulating DNMT enzymatic activity, however, are not fully elucidated. Here, we show that protein S-nitrosylation of a cysteine residue in DNMT3B attenuates DNMT3B enzymatic activity and consequent aberrant upregulation of gene expression. These genes include ...[more]

PMID: 36739439

Dataset Information

Pivotal role for S-nitrosylation of DNA methyltransferase in epigenetic regulation

Dataset's files

Publications

Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis.

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