Dataset Information

Characterization and proteomic analysis of plasma EVs recovered from healthy and diseased dogs with Canine Leishmaniosis

ABSTRACT: Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions like canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered using size-exclusion chromatography (SEC), EVs from 19 healthy and 20 CanL dogs’ plasma and performed proteomic analysis by LC-MS/MS to define their core proteomic composition and search for CanL-associated alterations. EVs-specific markers were identified in all preparations and also non-EVs proteins. Some EVs markers like CD82 were specific to the healthy animals, while others like the Integrin beta 3 were identified in most samples. The EVs-enriched preparations allowed the identification of 529 canine proteins that were identified in both groups, while 465 and 154 were only identified in healthy or CanL samples, respectively. A GO enrichment analysis revealed few CanL-specific terms. Leishmania spp. protein identifications were also found alt-hough with only 1 unique peptide. Ultimately, CanL-associated proteins of interest were identified and a core proteome was revealed that will be available for intra- and inter-species comparisons.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Leishmania Infantum Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Plasma

DISEASE(S): Leishmaniasis

SUBMITTER: Nuno Santarém

LAB HEAD: Anabela Cordeiro da Silva

PROVIDER: PXD039610 | Pride | 2023-05-10

REPOSITORIES: pride

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Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis.

Esteves Sofia S Lima Clara C Costa Inês I Osório Hugo H Fernandez-Becerra Carmen C Santarém Nuno N Cordeiro-da-Silva Anabela A

International journal of molecular sciences 20230313 6

Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions such as canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered, using ...[more]

PMID: 36982564

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