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After virus exposure, early bystander naïve CD8 T cell activation relies on NAD+ salvage metabolism

ABSTRACT: CD8 T cells play a central role in antiviral immunity. Type I interferons are among the earliest responders after virus exposure and can cause extensive reprogramming and antigen-independent bystander activation of CD8 T cells. Although bystander activation of pre-existing memory CD8 T cells is known to play an important role in host defense and immunopathology, its impact on naïve CD8 T cells remains underappreciated. Here we report that exposure to reovirus, both in vitro or in vivo, promotes bystander activation of naïve CD8 T cells within 24 hours and that this distinct subtype of CD8 T cell displays an innate, antiviral, type I interferon sensitized signature. The induction of bystander naïve CD8 T cells is STAT1 dependent and regulated through nicotinamide phosphoribosyl transferase (NAMPT)-mediated enzymatic actions within NAD+ salvage metabolic biosynthesis. These findings identify a novel aspect of CD8 T cell activation following virus infection with implications for human health and physiology.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, T Cell

DISEASE(S): Avian Influenza

SUBMITTER: Vishnupriyan Kumar

LAB HEAD: Shashi Gujar

PROVIDER: PXD039614 | Pride | 2023-01-30

REPOSITORIES: Pride

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			Action	DRS
	287366_a04945_NH_set1_Reo_mouse.mzid.gz	Mzid
	287367_a04946_NH_set1_Reo_mouse.mzid.gz	Mzid
	287368_a04947_NH_set1_Reo_mouse.mzid.gz	Mzid
	287369_a04948_NH_set1_Reo_mouse.mzid.gz	Mzid
	287370_a04949_NH_set1_Reo_mouse.mzid.gz	Mzid

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