Dataset Information

Quantitative proteomics reveals significant variation in host responses of cattle with differing buffalo fly susceptibility

ABSTRACT: This project aimed to characterise the immune response of cattle to buffalo fly infestation using cattle serum samples. The cattle were phenotyped into two groups, high buffalo fly burden and low buffalo fly burden cattle, following exposure to buffalo flies. The SWATH analysis was sued to measure the relative abundance of proteins in serum samples of the two groups at different time points.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Blood Serum

SUBMITTER: Ali Raza

LAB HEAD: Dr Peter

PROVIDER: PXD039655 | Pride | 2024-08-08

REPOSITORIES: Pride

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Publications

Quantitative proteomics reveals significant variation in host responses of cattle with differing buffalo fly susceptibility.

Kamran Muhammad M Raza Ali A Naseem Muhammad N MN Turni Conny C Tabor Ala E AE James Peter P

Frontiers in immunology 20240716

<h4>Background</h4>Control of buffalo flies (<i>Haematobia irritans exigua</i>, BFs) relies mainly on chemical methods; however, resistance to insecticides is widespread in BF populations. Breeding for resistance to BFs represents a possible alternative, but direct phenotyping of animals is laborious and often inaccurate. The availability of reliable diagnostic biomarker(s) to identify low BF carrier cattle would facilitate rapid and accurate selection for genetic improvement. However, limited i ...[more]

PMID: 39086482

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Project description:Puberty is a complex physiological event measured by various indicator traits in genetic improvement programs of beef cattle; thus, developing a more complete understanding of the genes and regulatory pathways and networks involved in puberty will provide knowledge to help improve genetic selection strategies. Herein, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed for cattle to achieve puberty (i.e., longissimus dorsi muscle, fat, and liver). These tissues were collected from pre (PRE)- and post (POST)-pubertal Brangus (3/8 Brahman; Bos indicus x 5/8 Angus; Bos taurus) heifers derived from a population of cattle used to identify QTL associated with fertility traits. In order to exploit the power of complementary omics analyses, PRE and POST puberty co-expression gene networks were constructed by combining the results from RNA-Seq, GWAS, and bovine transcription factors. RNA-Seq of 8 tissues among PRE and POST Brangus heifers revealed 1515 differentiallyexpressed and 943 tissue-specific genes within the 17,832 genes confirmed by metrics of RNA-Seq analysis. Combining the results from RNA-Seq and GWAS indentified a total of 25 QTL associated to heifer fertility. The hypothalamus experienced the most notable up-regulation of genes via puberty. Complementary omics procedures revealed 2,450 co-expressed genes across the 8 tissues relative to puberty. The PRE network had 372,861 connections whereas the POST network had 328,357 connections. A sub-network from this process revealed key transcriptional regulators (i.e., PITX2, FOXA1, TSG1D1, DACH2, LHX4, PROP1 and SIX6). Results from multiples sources of omics data will facilitate the design of breeding strategies to improve fertility in Bos indicus-influenced composite cattle. Sixty-one samples from PRE and POST pubertal composite beef heifers were analyzed with RNA-Seq. The transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to metabolism andbody morphometrics needed for cattle to achieve puberty (i.e.,) was characterized. These tissues were collected from pre (PRE)- and post (POST)-pubertal Brangus (3/8 Brahman x 5/8 Angus) heifers derived from a population of cattle used to identify QTL associated with fertility. Total RNA was purified using a Trizol protocol (Invitrogen, Carlsbad, CA). Sequencing libraries were made using TruSeq RNA Sample Preparation kit of Illumina (San Diego, CA).

Dataset Information

Quantitative proteomics reveals significant variation in host responses of cattle with differing buffalo fly susceptibility

Publications

Quantitative proteomics reveals significant variation in host responses of cattle with differing buffalo fly susceptibility.

Similar Datasets

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