Proteomics

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Bioaerosols in swine confinement buildings: A metaproteomic view

ABSTRACT: Swine confinement buildings (SCBs) represent workplaces with high biological air pollution. It is suspected that individual components of inhalable air are causatives of chronic respiratory disease that are regularly detected among workers. In order to understand the relationship between exposure and stress, the aim of this study was to develop a method to investigate the components of bioaerosols in more detail. For this purpose, bioaerosols from pig barns were collected on quartz filters from two exclusively housed pig types (porkers and gestating sows) and subsequently analyzed via a combinatorial approach of 16S rRNA amplicon sequencing and metaproteomics. The workflow helps to clarify diversity in bioaerosols from a taxonomic perspective, but also from a functional perspective.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

SUBMITTER: Susann Meyer  

LAB HEAD: PD Dr. Udo Jäckel

PROVIDER: PXD039685 | Pride | 2024-01-26

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20210105_Porkers_day2_Rep2.raw Raw
20210107_Porkers_day1_Rep1.raw Raw
20210107_Porkers_day2_Rep1.raw Raw
20210107_Porkers_day3_Rep1.raw Raw
20210107_Sows_day1_Rep1.raw Raw
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Bioaerosols in swine confinement buildings: A metaproteomic view.

Meyer Susann S   Hüttig Nicole N   Zenk Marianne M   Jäckel Udo U   Pöther Dierk-Christoph DC  

Environmental microbiology reports 20231102 6

Swine confinement buildings represent workplaces with high biological air pollution. It is suspected that individual components of inhalable air are causatives of chronic respiratory disease that are regularly detected among workers. In order to understand the relationship between exposure and stress, it is necessary to study the components of bioaerosols in more detail. For this purpose, bioaerosols from pig barns were collected on quartz filters and analysed via a combinatorial approach of 16S  ...[more]

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