Proteomics

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The secretome features associated with the development of re-sistance to vemurafenib in BRAFV600E mutated colon cancer cells


ABSTRACT: Patients with metastatic colorectal cancer (mCRC) carrying BRAFV600E mutation have worse response to chemotherapy and poor prognosis. The aim of this study was to conduct for the first time comparative proteomics profiling of the secretome from vemurafenib-sensitive vs. resistant colon cancer cells harboring BRAFV600E mutation in order to identify specific secretory features potentially associated with the development of resistance to vemurafenib. Towards this aim, we employed a label-free quantitative LC-MS/MS analysis. Obtained results pointed to aberrant regulation of DNA replication and the endoplasmic reticulum stress as the major secretome features associated with chemoresistant phenotype. Further studies are required to investigate diagnostic values of these proteins in the clinical setting and to examine their roles in acquired resistance to BRAF inhibition not only in colon cancer but also in other BRAF mutated cancers.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colorectal Adenocarcinoma Cell Line

DISEASE(S): Colorectal Adenocarcinoma,Colorectal Cancer

SUBMITTER: Antje Dittmann  

LAB HEAD: Mirela Sedic

PROVIDER: PXD039766 | Pride | 2023-04-29

REPOSITORIES: Pride

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Publications

Secretome Screening of BRAFV600E-Mutated Colon Cancer Cells Resistant to Vemurafenib.

Car Iris I   Dittmann Antje A   Klobučar Marko M   Grbčić Petra P   Kraljević Pavelić Sandra S   Sedić Mirela M  

Biology 20230417 4


Patients with metastatic colorectal cancer (mCRC) carrying BRAFV600E mutation have worse response to chemotherapy and poor prognosis. The BRAFV600E inhibitor vemurafenib has shown modest efficacy as monotherapy in BRAF-mutated mCRC due to the development of resistance. The aim of this study was to conduct a comparative proteomics profiling of the secretome from vemurafenib-sensitive vs. -resistant colon cancer cells harboring BRAFV600E mutation in order to identify specific secretory features po  ...[more]

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