Proteomics

Dataset Information

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Site-specific glycan analysis of ErbB2 in breast cancer cell BT474

ABSTRACT: This study provides site-specific profiles of asparagine-linked and serine/threonine-linked glycosylations on ErbB2, a therapeutic target RTK for ErbB2-positive cancer, through structural analysis of recombinant ErbB2 extracellular region and endogenous ErbB2 from ErbB2-positive breast cancer cells. Furthermore, comparison of the N-glycosylated structures of the extracellular regions of ErbB2 and its homologue, the epidermal growth factor receptor (EGFR), showed differences in the distribution and density of N-glycans on both molecules, providing new insights into the different activation mechanisms of ErbB2 and EGFR from a glycan perspective.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Naoki Fujitani  

LAB HEAD: Motoko Takahashi

PROVIDER: PXD039838 | Pride | 2024-05-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
BT474_IP.raw Raw
ErbB2.fasta Fasta
allPeptides.txt Txt
allSpectra.HCD.FTMS.iso_0.apl Other
allSpectra.HCD.FTMS.iso_1.apl Other
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Publications

Site-specific glycosylation analysis of epidermal growth factor receptor 2 (ErbB2): exploring structure and function toward therapeutic targeting.

Fujitani Naoki N   Uehara Yasuaki Y   Ariki Shigeru S   Hashimoto Ukichiro U   Mukai Jo J   Hasegawa Yoshihiro Y   Takahashi Motoko M  

Glycobiology 20240401 3

Glycans found on receptor tyrosine kinases (RTKs) have emerged as promising targets for cancer chemotherapy, aiming to address issues such as drug resistance. However, to effectively select the target glycans, it is crucial to define the structure and function of candidate glycans in advance. Through mass spectrometric analysis, this study presents a "glycoform atlas" of epidermal growth factor receptor 2 (ErbB2), an RTK targeted for the treatment of ErbB2-positive cancers. Our analysis provides  ...[more]

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