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Identification of TTC29 protein partners in mouse testis

ABSTRACT: We previously identified gene variants in TTC29 that induce human male infertility due to asthenozoospermia and MMAF phenotype (Lores et al. Am J hum genet 2019). By means of CRISPR/Cas9 mutagenesis we demonstrated that the orthologous TTC29 protein in mouse also constitute a flagellar protein that is required for proper beating of the flagella, indicating an evolutionary-conserved function of TTC29. In here, we took advantage of the mutant mouse model to further decipher the protein complexes associated with TTC29 function within the flagella. We performed co-immunoprecipitation experiments on testis protein extracts from wild-type mice using TTC29 specific antibodies and performed comparative mass spectrometry analyses with two TTC29 KO mouse lines we previously generated and shown to lack TTC29 protein (Ttc29-/- L5 and L7) (Lores et al. Am J hum genet 2019. Mass spectrometry analyses were performed on four independent experimental replicates for each mouse line.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis

SUBMITTER: Johanna BRUCE

LAB HEAD: Patrick LORES

PROVIDER: PXD039854 | Pride | 2023-11-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AmTO200303_TTC29_Ip_107_F36176_200702093028.raw	Raw
	AmTO200303_TTC29_Ip_108_F36177_200702105505.raw	Raw
	AmTO200303_TTC29_Ip_116_F36178_200702121942.raw	Raw
	AmTO200706_TTC29_Ip_135_L5_F36716.raw	Raw
	AmTO200706_TTC29_Ip_152_L7_F36719.raw	Raw

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Identification of TTC29 protein partners in mouse testis

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