Dataset Information

Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6

ABSTRACT: Cellular selectivity of a chemical probe for the UBD of HDAC6. A biotinylated analog of HDAC6 chemical probe SGC-UBD253 bound to streptavidin beads was used to enrich HDAC6 from cytoplasmic fraction of HEK293 lysate. A triplicate of each condition - competition with probe, control, DMSO - was done.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Early Embryonic Cell

SUBMITTER: Suzanne Ackloo

LAB HEAD: Cheryl H Arrowsmith

PROVIDER: PXD039880 | Pride | 2023-10-24

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	39946_DMSO_BR1_NBCC161_Arrowsmith_Lumos_quart.raw	Raw
	39947_DMSO_BR2_NBCC161_Arrowsmith_Lumos_quart.raw	Raw
	39948_DMSO_BR3_NBCC161_Arrowsmith_Lumos_quart.raw	Raw
	39949_Control_BR1_NBCC161_Arrowsmith_Lumos_quart.raw	Raw
	39950_Control_BR2_NBCC161_Arrowsmith_Lumos_quart.raw	Raw

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Publications

Discovery and Characterization of a Chemical Probe Targeting the Zinc-Finger Ubiquitin-Binding Domain of HDAC6.

Harding Rachel J RJ Franzoni Ivan I Mann Mandeep K MK Szewczyk Magdalena M MM Mirabi Bijan B Ferreira de Freitas Renato R Owens Dominic D G DDG Ackloo Suzanne S Scheremetjew Alexej A Juarez-Ornelas Kevin A KA Sanichar Randy R Baker Rachel J RJ Dank Christian C Brown Peter J PJ Barsyte-Lovejoy Dalia D Santhakumar Vijayaratnam V Schapira Matthieu M Lautens Mark M Arrowsmith Cheryl H CH

Journal of medicinal chemistry 20230727 15

Histone deacetylase 6 (HDAC6) inhibition is an attractive strategy for treating numerous cancers, and HDAC6 catalytic inhibitors are currently in clinical trials. The HDAC6 zinc-finger ubiquitin-binding domain (UBD) binds free C-terminal diglycine motifs of unanchored ubiquitin polymer chains and protein aggregates, playing an important role in autophagy and aggresome assembly. However, targeting this domain with small molecule antagonists remains an underdeveloped avenue of HDAC6-focused drug d ...[more]

PMID: 37499118

Dataset Information

Discovery and characterization of a chemical probe targeting the zinc-finger ubiquitin-binding domain of HDAC6

Dataset's files

Publications

Discovery and Characterization of a Chemical Probe Targeting the Zinc-Finger Ubiquitin-Binding Domain of HDAC6.

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