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Immunoprecipitation mass spectrometry of SPARK-mNG

ABSTRACT: SPARK is an AGC kinase found specifically in apicomplexan parasites. We have previously shown that SPARK is essential for parasite calcium mobilization, egress, and invasion. Here we have performed immunoprecipitation followed by mass spectrometry to determine interaction partners of SPARK. We identify a hypothethical protein that interacts with SPARK, which we term SPARKEL.

INSTRUMENT(S): Orbitrap Exploris 480, FAIMS compensation voltage

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039919 | Pride | 2024-07-12

REPOSITORIES: Pride

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