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Proximity labeling of SPARKEL-TurboID

ABSTRACT: Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing SPARKEL-TurboID endogenously.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039985 | Pride | 2024-07-12

REPOSITORIES: Pride

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			Action	DRS
	20220729_tgALH184_intracellular_DDA_90min.raw	Raw
	20220729_tgALH294_intracellular_DDA_90min.raw	Raw
	20220731_tgALH184_intracellular_DDA_90min.raw	Raw
	20220731_tgALH294_intracellular_DDA_90min.raw	Raw
	Aug2022_tgALH294_DDA_2reps_90min.mzML	Mzml

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Proximity labeling of TurboID-SPARKEL

Project description:Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing TurboID-SPARKEL endogenously.

2024-07-12 | PXD039986 | Pride

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Project description:SPARK is an AGC kinase found in apicomplexan parasites. In previous work, we showed that SPARK knockdown leads to inefficient T. gondii calcium mobilization, egress, and invasion of host cells. We performed immunoprecipitation and mass spectrometry to determine the interaction partners of SPARK-mNG-mAID isolated from T. gondii lysates. We identified a strong interaction partner, which we term SPARKEL.

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