RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment
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ABSTRACT: Lassa virus is a negative-strand RNA virus with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming the ribonucleoparticles together with the viral RNA and the L protein, which have to be continuously restructured during viral genome replication and transcription. Z protein is important for ribonucleoparticle recruitment, viral particle assembly and budding, and has also been shown to interact with the L protein. However, the interaction of NP, viral RNA and Z is poorly understood. Here, we characterize the interactions between Lassa virus NP, Z and RNA using structural mass spectrometry. We identify the presence of RNA as the driver for disassembly of ring-like NP trimers into monomers to subsequently form higher order assemblies. We locate the interaction site of Z and NP and demonstrate that while NP binds Z independently of the presence of RNA, this interaction is pH-dependent. These data improve our understanding of ribonucleoparticle assembly and recruitment.
INSTRUMENT(S): Q-Tof Ultima, Q Exactive HF
ORGANISM(S): Lassa Mammarenavirus
SUBMITTER: Lennart Sänger
LAB HEAD: Charlotte Uetrecht
PROVIDER: PXD040186 | Pride | 2023-12-18
REPOSITORIES: Pride
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