Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture, Lymphoblast, Fibroblast
SUBMITTER: Ryan Kelly
LAB HEAD: Ryan T. Kelly
PROVIDER: PXD040455 | Pride | 2023-07-11
REPOSITORIES: Pride
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10ng_A549_H_Iso16_30CountFromKit.raw | Raw | |||
10ng_A549_L_Iso16_30CountFromKit.raw | Raw | |||
10ng_HeLa_H_Iso16_30CountFromKit.raw | Raw | |||
10ng_HeLa_L_Iso16_30CountFromKit.raw | Raw | |||
Chip1_K562HeLa_F2.raw | Raw |
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Analytical chemistry 20230511 20
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted in dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods have been separately applied to SCP to increase throughput through multiplexing. Here we combined both forms of labeling to achieve multiplicative scaling for higher throughput. Two-plex stable isotope labeling of amino acids in cell culture (SILAC) and isobaric ta ...[more]