Proteomics

Dataset Information

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Molecular signature associated with cladribine treatment in multiple sclerosis patients


ABSTRACT: Little is known about the molecular profiling associated with the effect of cladribine in patients with multiple sclerosis (MS). Here, we aimed first to characterize the transcriptomic and proteomic profiles induced by cladribine in blood cells, and second to identify potential treatment response biomarkers to cladribine in patients with MS. PBMCs treated in vitro with cladribine were characterized by a major downregulation of gene, protein, and miRNA expression compared with the untreated cells. An intermediate pattern between the cladribine-treated and untreated conditions was observed in PBMCs treated with cladribine in its inactive form. The differential expression analysis of each dataset led to the identification of four genes and their encoded proteins, and twenty-two miRNAs regulating their expression, that were associated with cladribine treatment. Two of these genes (PPIF and NHLRC2), and three miRNAs (miR-21-5p, miR-30b-5p, and miR-30e-5p) were validated ex vivo in MS patients treated with cladribine. Conclusions: By using a combination of omics data and bioinformatics approaches we were able to identify a multiomics molecular profile induced by cladribine in vitro in PBMCs. We also identified a number of biomarkers that were validated ex vivo in PBMCs from MS patients treated with cladribine that have the potential to become treatment response biomarkers to this drug.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Peripheral Blood Mononuclear Cell, Blood

DISEASE(S): Multiple Sclerosis

SUBMITTER: Eva Borràs  

LAB HEAD: Eduard Sabidó

PROVIDER: PXD040572 | Pride | 2024-08-14

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
2020MQ013_NIFI.pdResult Other
2020MQ013_NIFI_001_P1-1_02_1ug.raw Raw
2020MQ013_NIFI_002_P1-2_01_1ug.raw Raw
2020MQ013_NIFI_003_P1-3_01_1ug.raw Raw
2020MQ013_NIFI_004_P1-4_01_1ug.raw Raw
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