Dataset Information

Proteome characterization of HCMV virus microenvironment with addition of cidofovir

ABSTRACT: The control of host processes which influence cell-cell communication is central to determining the outcome of a virus infection in tissue. Despite this, it remains unclear how cells either in close or distant proximity to an infected cell differentially respond to the primary infection. Here, we established a virus infection microenvironment to resolve molecular features and functional consequences of cell spatial address within this localized niche by mass spectrometry. To rule out the possibility that neighboring cells are infected by the viral progeny from the primary infection, cidofovir is added in the system to prevent viral genome replication in order to prevent the spread of infection.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Bokai Song

LAB HEAD: Ileana Cristea

PROVIDER: PXD040582 | Pride | 2023-05-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	2023.2.15_VME_CDV.msf	Msf
	2023.2.15_VME_CDV_consensus.pdResult	Other
	20230209_HCMV_CDV_VME_Distal_BR1.raw	Raw
	20230209_HCMV_CDV_VME_Distal_BR2.raw	Raw
	20230209_HCMV_CDV_VME_Distal_BR3.raw	Raw

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Publications

Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.

Song Bokai B Sheng Xinlei X Justice Joshua L JL Lum Krystal K KK Metzger Peter J PJ Cook Katelyn C KC Kostas James C JC Cristea Ileana M IM

Science advances 20230510 19

Communication between infected cells and cells in the surrounding tissue is a determinant of viral spread. However, it remains unclear how cells in close or distant proximity to an infected cell respond to primary or secondary infections. We establish a cell-based system to characterize a virus microenvironment, distinguishing infected, neighboring, and distal cells. Cell sorting, microscopy, proteomics, and cell cycle assays allow resolving cellular features and functional consequences of proxi ...[more]

PMID: 37163594

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