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Comparison of gel and urea methods of digestion for proteomic analysis of organ perfusion solutions

ABSTRACT: The aim of this study was to develop an optimized Shotgun proteomics method for perusate by comparing and identifying the most effective and reliable techniques for protein estimation and digestion.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sadr Shaheed

LAB HEAD: Dr Sadr ul Shaheed

PROVIDER: PXD040856 | Pride | 2024-01-26

REPOSITORIES: Pride

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			Action	DRS
	In-gel-kidney-HMP-02-P1-FD09177341.raw	Raw
	In-gel-kidney-HMP-02-P2-FD09177356.raw	Raw
	In-gel-kidney-HMP-02-P2-FD09177913.raw	Raw
	In-gel-kidney-HMP-02-P3-FD01269744.raw	Raw
	In-gel-kidney-HMP-02-P3-FD06420623.raw	Raw

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Publications

Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions.

Snashall Corinna M CM Sutton Chris W CW Faro Letizia Lo LL Ceresa Carlo C Ploeg Rutger R Shaheed Sadr Ul SU

Clinical proteomics 20231115 1

<h4>Purpose</h4>The organ perfusion solution (perfusate), collected at clinically and temporally significant stages of the organ preservation and transplantation process, provides a valuable insight into the biological status of an organ over time and prior to reperfusion (transplantation) in the recipient. The objective of this study was to assess two bottom-up proteomics workflows for the extraction of tryptic peptides from the perfusate.<h4>Experimental design</h4>Two different kinds of perfu ...[more]

PMID: 37968584

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Perfluorooctanoate in Aqueous Urea Solutions: Micelle Formation, Structure, and Microenvironment.

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