Dataset Information

Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry

ABSTRACT: We propose here a workflow based on an automated immunocapture followed by top-down and middle-down LC-MS/MS approaches to characterize mAb proteoforms spiked in mouse plasma. We demonstrate the applicability of our workflow on a large concentration range using pembrolizumab as a model. We also compare the performance of two state-of-the-art Orbitrap platforms (Tribrid Eclipse and Exploris 480) for these studies. The added value of our workflow for an accurate and sensitive characterization of mAb proteoforms in mouse plasma is highlighted.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Blood Plasma

SUBMITTER: Jonathan Dhenin

LAB HEAD: Julia Chamot-Rooke

PROVIDER: PXD040896 | Pride | 2024-08-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	01_20220805_JD_Pmab_PD1_0-5ugmL_7-5K_Chase_01.raw	Raw
	01_20220810_JD_Pmab_PD1_IdeS-Reduction_0-5ugmL_7-5K_01.raw	Raw
	01bis_20220802_JD_Pmab_PD1_0-5ugmL_7-5K_01.raw	Raw
	02_20220721_E_JD_Pmab_PD1_0-5ugmL_R01.raw	Raw
	02_20220802_JD_Pmab_PD1_0-5ugmL_7-5K_02.raw	Raw

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Monitoring mAb proteoforms in mouse plasma using an automated immunocapture combined with top-down and middle-down mass spectrometry.

Dhenin Jonathan J Lafont Valérie V Dupré Mathieu M Krick Alain A Mauriac Christine C Chamot-Rooke Julia J

Proteomics 20230721 3-4

Monoclonal antibodies (mAbs) have established themselves as the leading biopharmaceutical therapeutic modality. Once the developability of a mAb drug candidate has been assessed, an important step is to check its in vivo stability through pharmacokinetics (PK) studies. The gold standard is ligand-binding assay (LBA) and liquid chromatography-mass spectrometry (LC-MS) performed at the peptide level (bottom-up approach). However, these analytical techniques do not allow to address the different mA ...[more]

PMID: 37480175

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