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VEGFR2 pY1173 binding partners as identified by shotgun proteomics

ABSTRACT: The main goal of the project was to identify proteins binding to vascular endothelial growth factor receptor 2 (VEGFR2) phosphorylation site Y1173. Synthetic peptides, phosphorylated or not, covering different tyrosine phosphorylation sites in VEGFR2 were immobilized and incubated with cell lysates from human umbilical vein endothelial cells. Retained proteins were analyzed by mass spectrometry. Proteins specifically binding to pY1173 peptide were categorized with regard to the presence of an Src Homology 2 (SH2) domain and the main hits were validated in intact cells treated or not with VEGF, for their ability to bind to the activated wild type VEGFR2 but not to a mutant Y1173F VEGFR2. The role of the pY1173 binding partners in VEGF-regulated endothelial biology was further examined in vitro and in vivo.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Georgios Mermelekas

LAB HEAD: Janne Lehtiö

PROVIDER: PXD041024 | Pride | 2023-09-19

REPOSITORIES: Pride

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			Action	DRS
	20180403_GMLCWES_3hrgrad_5ul_1.raw	Raw
	20180403_GMLCWES_3hrgrad_5ul_2.raw	Raw
	20180403_GMLCWES_3hrgrad_5ul_3.raw	Raw
	20180403_GMLCWES_3hrgrad_5ul_4.raw	Raw
	20180403_GMLCWES_3hrgrad_5ul_5.raw	Raw

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