Dataset Information

Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide

ABSTRACT: Desmocollin-1 (DSC1) is a desmosomal transmembrane glycoprotein that maintains cell-to-cell adhesion. DSC1 was previously associated with lymph node metastasis of luminal A breast tumors and was found to increase metastatic potential of MCF7 cells in vitro. To delineate DSC1 role in breast cancer metastasis and evaluate possibilities of DSC1 modulation, we investigated the effect of DSC1 overexpression on morphology, cell survival, transcriptome, proteome and interactome of MCF7 cells, a luminal A breast cancer model, stably transduced with lentiviral vector carrying DSC1 gene (MCF7-DSC1-GFP). We moreover identified inhibitor parthenolide to decrease DSC1 protein levels and to modulate the molecular mechanisms associated with DSC1 in MCF7 cells. This PRIDE project includes quantitative analysis results for the total proteome LC-DIA-MS/MS experiment evaluating DSC1 overexpression and parthenolide treatment in MCF7 breast cancer cell line, and results of pulldown analysis of DSC1-interacting proteins in MCF7 cells with and without parthenolide treatment.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Pavel Bouchal

LAB HEAD: Pavel Bouchal

PROVIDER: PXD041029 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200310_145748_DSC1_pulldowns_inh_3877_2003.sne	Other
	3590_Lumik1_130m_DIA01_DG1.raw	Raw
	3590_Lumik1_130m_DIA01_DG1_P.raw	Raw
	3590_Lumik1_130m_DIA01_DG2.raw	Raw
	3590_Lumik1_130m_DIA01_DG2_P.raw	Raw

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Publications

Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide.

Lapcik Petr P Sulc Petr P Janacova Lucia L Jilkova Katerina K Potesil David D Bouchalova Pavla P Müller Petr P Bouchal Pavel P

Cellular & molecular biology letters 20230824 1

<h4>Background</h4>Desmocollin-1 (DSC1) is a desmosomal transmembrane glycoprotein that maintains cell-to-cell adhesion. DSC1 was previously associated with lymph node metastasis of luminal A breast tumors and was found to increase migration and invasion of MCF7 cells in vitro. Therefore, we focused on DSC1 role in cellular and molecular mechanisms in luminal A breast cancer and its possible therapeutic modulation.<h4>Methods</h4>Western blotting was used to select potential inhibitor decreasing ...[more]

PMID: 37620794

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