Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A Treg specific long non-coding RNA maintains immune-metabolic homeostasis in ageing liver

ABSTRACT: To find specific transcription factors (TFs) interact with Altre to regulate target gene expression by Cis- or Trans- action on chromatin.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell

SUBMITTER: Chenbo Ding

LAB HEAD: Chenbo Ding

PROVIDER: PXD041040 | Pride | 2023-05-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Altre-Treg_MS.xlsx	Xlsx
	XQ-S.zip	Other
	XQ-S1.raw	Raw
	XQ-S1.raw.md5	Raw
	XQ-S2.raw	Raw

Items per page:

1 - 5 of 6

Similar Datasets

Gene expression analysis of iTregs and nTregs

Project description:MBP TCR Tg mouse (1B3) generates only iTregs when crossed onto RAGKO background. Tregs from 1B3.RAG mouse were used for gene expression analsysis to determine genes selectively expressed in peripherally generated iTregs iTregs from 1B3.RAG mice (>90% Foxp3+) were run against total Tregs from 1B3 and WT NOD mice. Foxp3- CD4+T cells were used as control from all strains as Tconv. (1B3 denotes MBP-TCR Tg mouse).

2013-07-03 | E-GEOD-39724 | biostudies-arrayexpress

RNA-seq of FOXP3+ CD4SP regulatory T cells and their FOXP3- CD4SP conventional counterparts from the human thymus

Project description:Generation of transcriptomes to provide in-depth expression profiles of tTreg and tTconv and to obtain the differential expression signature defining thymic Tregs in humans.

2024-10-30 | E-MTAB-11211 | biostudies-arrayexpress

Expression of CD39 and CD45RO on CD4+CD25hi T cells identifies human naïve (G3) and effector Treg (G2), and distinguishes them from activated non-Treg cells (Tresp).

Project description:Human responder T-cells (Tresp) and 2 lineages of regulatory T cells (Tregs), namely G2 and G3, were isolated by flow sorting based on their immunoreacitivities against specific cell surface markers and their gene expression profiles were interrogated using Affymetrix Exon 1.0 ST Arrays Comparison of gene expression profiles between responder T-cells, G2 Tregs and G3 Tregs

2013-10-01 | E-GEOD-47667 | biostudies-arrayexpress

The lipid metabolism enzyme DGAT1 mediates Treg control of type 2 immunity at barrier sites

Project description:Regulatory T cells were sorted from the colon of mice under steady state conditions from mice that were either wild type or Dgat1 knockout. To gain enough cells for sequencing samples from the same group were pooled (n=5/pool).

2023-01-01 | E-MTAB-9477 | biostudies-arrayexpress

Human Treg LC-MSMS - Foxp3 drives oxidative phosphorylation and protection from lipotoxicity

Project description:Regulatory T cells (Treg) have been shown to adopt a catabolic metabolic programme with increased capacity for fatty acid oxidation fuelled oxidative phosphorylation (OXPHOS). The role of Foxp3 in this metabolic shift is poorly understood. Here we show that Foxp3 was sufficient to induce a significant increase in the spare respiratory capacity of the cell, the extra OXPHOS capacity available to a cell to meet increased demands on energy in response to work. Foxp3-expressing cells were enhanced in their ability to utilise palmitate for respiration and, in addition, the activity of electron transport complexes I, II and IV were enhanced following Foxp3 expression. Foxp3 also imparts a selective advantage in ATP generation capacity, one that might be exploited as a source of adenosine for functional immunomodulation. In order to explore possible mechanisms for these differences in metabolism we conducted a quantitative proteomics study to compare the contribution of TGFβ and the transcription factor Foxp3 to the Treg proteome. We used quantitative mass spectrometry to examine differences between proteomes of nuclear and cytoplasmic Foxp3-containing CD4+ T cells from various sources with Foxp3- activated CD4 T cells, as well as Treg from human peripheral blood. Gene set enrichment analysis of our proteomic datasets demonstrated that Foxp3 expression is associated with a significant up regulation of several members of the mitochondrial electron transport chain. Not only does Foxp3 influence genes directly concerned with immune function, but also with the energy generating functions of Treg.

2017-02-15 | PXD001906 | Pride

CD4+ T-cell sub-populations

Project description:We investigated the transcriptome of closely related CD4+ T-cell population from healthy human donors to elucidate the processes that underly their specialized immune functions

2014-12-31 | E-MTAB-1603 | biostudies-arrayexpress

Whole genome expression analysis of tumor regulatory T cells expressing interleukin-10

Project description:The tumor microenvironment contains high frequencies of inflammatory regulatory T (Treg) cells. These Treg exhibit superior regulatory function compared with those from other environments such as the spleen, partially due to expression of anti-inflammatory interleukin-10. In order to gain insight into the origins and functional roles of different Treg subsets, we used whole genome microarray analysis to characterize tumor IL-10+ and tumor IL-10- Treg subsets obtained from VERT-X reporter mice bearing transplantable tumors, along with total tumor Treg and spleen Treg from FoxP3-EGFP reporter mice. Few genes were found to differ between IL-10+ and IL-10- tumor Treg subsets (29 upregulated, 88 downregulated), suggesting a common origin of each Treg subset. The specific gene expression profile of IL-10+ tumor Treg was associated with the tumor microenvironment and absent from spleen Treg, suggesting it to be driven by components of the inflammatory tumor microenvironment. The IL-10+ tumor Treg gene expression profile displayed upregulation of genes associated with a higher activation state and greater effector function. Pooled MC38 tumor tissue from VERT-X or FoxP3-EGFP reporter mice were used to obtain IL10+ tumor Treg (VERT-X), IL10- tumor Treg (VERT-X) and total tumor Treg (FoxP3-EGFP). Spleens from tumor-free FoxP3-EGFP mice were used for spleen Treg. Three experiments for each population gave a total 12 RNA samples.

2013-08-31 | E-GEOD-49380 | biostudies-arrayexpress

RNAseq of bulk regulatory T cells (Tregs) and conventional T cells (Tconvs) during acute kidney injury and fibrosis development

Project description:We performed bulk RNA-seq from FACS-sorted Tregs (Foxp3+) and T convs (Foxp3-) CD4+ T cells from kidneys and spleens at different phases after injury.

2020-01-31 | E-MTAB-7961 | biostudies-arrayexpress

ATAC-seq of FOXP3+ CD4SP regulatory T cells and their FOXP3- CD4SP conventional counterparts from the human thymus

Project description:Characterisation of genome-wide chromatin accessibility (tn5-digested, nucleosome depleted) from thymic Treg and thymic Tconv and to obtain the gene regulatory networks defining thymic Tregs in humans.

2024-10-14 | E-MTAB-11220 | biostudies-arrayexpress