Proteomics

Dataset Information

0

Human fibroblast-like synoviocytes (FLSs) -derived secretome


ABSTRACT: Invasive pannus, mainly composed of fibroblast-like synoviocytes (FLSs), is a hallmark of rheumatoid arthritis (RA) pathology. Secreted proteins from RA-FLS play key roles in RA invasive pannus. However, RA-FLS-derived secretome associated with invasive pannus has not been systematically investigated. Here, we first identified 843 secreted proteins from RA-FLSs treated with TNFα and IL-1β using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Functional enrichment analysis revealed that 58.5% (493 proteins) of the secretome were associated with pannus-driven RA pathologies. Among them, parallel reaction monitoring (PRM) analysis then identified 16 secreted proteins that were increased in RA SFs (117 samples) than in OA SFs (45 samples). Of them, MYH9 further showed significant positive correlations with RA pathological parameters, such as synovial hyperplasia, increased articular vascularity, and inflammation severity. Molecular and cellular experiments confirmed that MYH9 was expressed in RA-FLSs and more highly under disease-aggravating conditions, and MYH9 depletion significantly defected migration and invasion of RA-FLS in RA pannus. Our study provides a comprehensive resource of RA-FLS-derived secretome, and our results suggest MYH9 that was increased in RA SF and strongly correlated with disease severity as a potential therapeutic target for invasive pannus.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Sehyun Chae  

LAB HEAD: Sehyun Chae

PROVIDER: PXD041077 | Pride | 2023-06-30

REPOSITORIES: Pride

Dataset's files

Source:
altmetric image

Publications

Identification of MYH9 as a key regulator for synoviocyte migration and invasion through secretome profiling.

Lee Saseong S   Choi Eunbyeol E   Chae Sehyun S   Koh Jung Hee JH   Choi Yoolim Y   Kim Jung Gon JG   Yoo Seung-Ah SA   Hwang Daehee D   Kim Wan-Uk WU  

Annals of the rheumatic diseases 20230515 8


<h4>Objectives</h4>'Invasive pannus' is a pathological hallmark of rheumatoid arthritis (RA). This study aimed to investigate secretome profile of synovial fibroblasts of patients with RA (RA-FLSs), a major cell type comprising the invasive pannus.<h4>Methods</h4>Secreted proteins from RA-FLSs were first identified using liquid chromatography-tandem mass spectrometry analysis. Ultrasonography was performed for affected joints to define synovitis severity at the time of arthrocentesis. Expression  ...[more]

Similar Datasets

2014-01-09 | E-GEOD-49604 | biostudies-arrayexpress
2020-04-18 | E-MTAB-8873 | biostudies-arrayexpress
2013-04-25 | E-GEOD-46364 | biostudies-arrayexpress
2014-01-09 | GSE49604 | GEO
2018-03-15 | E-MTAB-6457 | biostudies-arrayexpress
2024-04-18 | PXD051384 | Pride
2012-05-13 | E-GEOD-31667 | biostudies-arrayexpress
2022-08-17 | GSE200030 | GEO
2015-01-14 | E-GEOD-64922 | biostudies-arrayexpress
2018-02-12 | GSE65908 | GEO