Dataset Information

Palaeoproteomics of archaeological shell beads

ABSTRACT: In this project we have applied palaeoproteomics to study archaeological shell beads dating back to the beginning of the 7th mill. cal BCE and recovered from a prehistoric site in Jordan (Ba'ja). These beads were made of mollusc shells and were of unknown origin, but suspected to be made of larger bivalves such as Tridacna or Spondylus sp. The aim of the study was to analyse ancient proteins extracted from these beads in order to infer their biological origin. Tiny amounts of ancient samples were used and the extracted proteins were analysed by high resolution liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). In order to identify obtained peptides, we have created an Ã¢â‚¬Ëœin-houseÃ¢â‚¬â„¢ molluscan protein database, by gathering molluscan sequences (proteins and transcriptomes), which were downloaded from publicly available databases in NCBI. Searching against this comprehensive database, we were able to identify a number of shell proteins from Tridacna sp., the giant clam shells. Our study reports the oldest molluscan protein sequences ever recovered that come from a very warm environment.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mollusca

SUBMITTER: Jorune Sakalauskaite

LAB HEAD: Matthew J. Collins

PROVIDER: PXD041082 | Pride | 2024-11-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210705_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_labblank_JS_Palto.raw	Raw
	20210705_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_labblank_JS_Palto.refined.mgf	Mgf
	20210705_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_mollusk_Palto499.raw	Raw
	20210705_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_mollusk_Palto499.refined.mgf	Mgf
	20210705_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_mollusk_Palto500.raw	Raw

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Publications

Threads of memory: Reviving the ornament of a dead child at the Neolithic village of Ba`ja (Jordan).

Alarashi Hala H Benz Marion M Gresky Julia J Burkhardt Alice A Fischer Andrea A Gourichon Lionel L Gerlitzki Melissa M Manfred Martin M Sakalauskaite Jorune J Demarchi Beatrice B Mackie Meaghan M Collins Matthew M Odriozola Carlos P CP Garrido Cordero José Ángel JÁ Avilés Miguel Ángel MÁ Vigorelli Luisa L Re Alessandro A Gebel Hans Georg K HGK

PloS one 20230802 8

In 2018, a well-constructed cist-type grave was discovered at Ba`ja, a Neolithic village (7,400-6,800 BCE) in Southern Jordan. Underneath multiple grave layers, an 8-year-old child was buried in a fetal position. Over 2,500 beads were found on the chest and neck, along with a double perforated stone pendant and a delicately engraved mother-of-pearl ring discovered among the concentration of beads. The first was found behind the neck, and the second on the chest. The meticulous documentation of t ...[more]

PMID: 37531349

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