Dataset Information

Easy, robust, and reproducible online acid cleavage of proteins in mobile phase for fast quantitative LC-MS bottom-up proteomics – application for ricin detection

ABSTRACT: The data were recorded during the development, optimization, and characterization of a method for fast online acid cleavage of proteins directly in the mobile phase.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human) Ricinus Communis

SUBMITTER: Juraj Lenco

LAB HEAD: Juraj Lenco

PROVIDER: PXD041124 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	DN186_Trastuzumab_C_reduced_1i0uGuL_pyroLC_01.raw	Raw
	DN186_Trastuzumab_C_reduced_1i0uGuL_pyroLC_01.raw_20230216.xlsx	Xlsx
	DN186_Trastuzumab_C_reduced_1i0uGuL_pyroLC_02.raw	Raw
	DN186_Trastuzumab_C_reduced_1i0uGuL_pyroLC_02.raw_20230216.xlsx	Xlsx
	DN186_Trastuzumab_C_reduced_1i0uGuL_pyroLC_03.raw	Raw

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Publications

Easy, Robust, and Repeatable Online Acid Cleavage of Proteins in Mobile Phase for Fast Quantitative LC-MS Bottom-Up Protein Analysis─Application for Ricin Detection.

Naplekov Denis K DK Jadeja Siddharth S Fučíková Alena Myslivcová AM Švec František F Sklenářová Hana H Lenčo Juraj J

Analytical chemistry 20230811 33

Sample preparation involving the cleavage of proteins into peptides is the first critical step for successful bottom-up proteomics and protein analyses. Time- and labor-intensiveness are among the bottlenecks of the commonly used methods for protein sample preparation. Here, we report a fast online method for postinjection acid cleavage of proteins directly in the mobile phase typically used for LC-MS analyses in proteomics. The chemical cleavage is achieved in 0.1% formic acid within 35 s in a ...[more]

PMID: 37565982

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