Proteomics

Dataset Information

0

Sirtuin 2 Promotes HCMV Replication by Regulating Cell Cycle Progression

ABSTRACT: The host cell cycle is a major target for manipulation by viral pathogens, which often replicate in certain cell cycle stages. The prevalent pathogen human cytomegalovirus (HCMV), for instance, only replicates during G1 and therefore employs diverse mechanisms for regulating cell cycle progression. Here, we demonstrate that the human enzyme sirtuin 2 (SIRT2) is required for HCMV-mediated cell cycle dysregulation. By combining virology assays with mass pectrometry-based proteome, acetylome, and interactome analyses, we have defined a pro-virus function for SIRT2 in regulating the G1 to S phase transition.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

SUBMITTER: Cora Betsinger  

LAB HEAD: Ileana M. Cristea

PROVIDER: PXD041171 | Pride | 2023-10-13

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20220319_CNB_SIRT2-IP_0HPI_R1_150.raw Raw
20220319_CNB_SIRT2-IP_0HPI_R2_150.raw Raw
20220319_CNB_SIRT2-IP_0HPI_R3_150.raw Raw
20220319_CNB_SIRT2-IP_12HPI_R1_150.raw Raw
20220319_CNB_SIRT2-IP_12HPI_R2_150.raw Raw
Items per page:
1 - 5 of 69
altmetric image

Publications

Sirtuin 2 promotes human cytomegalovirus replication by regulating cell cycle progression.

Betsinger Cora N CN   Justice Joshua L JL   Tyl Matthew D MD   Edgar Julia E JE   Budayeva Hanna G HG   Abu Yaa F YF   Cristea Ileana M IM  

mSystems 20231102 6

<h4>Importance</h4>This study expands the growing understanding that protein acetylation is a highly regulated molecular toggle of protein function in both host anti-viral defense and viral replication. We describe a pro-viral role for the human enzyme SIRT2, showing that its deacetylase activity supports HCMV replication. By integrating quantitative proteomics, flow cytometry cell cycle assays, microscopy, and functional virology assays, we investigate the temporality of SIRT2 functions and sub  ...[more]

Similar Datasets

Proteomics Sirt2 inhibition reprograms T cell metabolism for optimal anti-tumor immunity: Sirt2 Interacting Proteins 2
2020-07-03 | PXD020150 | Pride
Proteomics Sirt2 inhibition reprograms T cell metabolism for optimal anti-tumor immunity: Sirt2 Interacting Proteins
2020-06-15 | PXD012811 | Pride
Proteomics SIRT2 interactomics in HeLa cells
2023-12-05 | PXD045456 | Pride
Proteomics Proteomics-based identification of SIRT2 interaction partners
2020-02-20 | PXD011523 | Pride
Proteomics CRISPR-Cas9 genetic screen leads to the discovery of L-Moses, a KAT2B inhibitor that attenuates Tunicamycin-mediated neurodegeneration
2023-03-10 | PXD038395 | Pride
Proteomics Fine-scale adaptive divergence and plasticity revealed by genetic and phenotypic variation in a marine invertebrate
2018-07-27 | PXD008057 | Pride
Proteomics Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis
2018-10-26 | PXD006036 | Pride
Proteomics High intensity interval training remodels the proteome and acetylome in human skeletal muscle
2022-06-09 | PXD023084 | Pride
Proteomics Sirt2 inhibition reprograms T cell metabolism for optimal anti-tumor immunity: Lysine Acetylation
2020-06-15 | PXD012796 | Pride
Proteomics Deacetylation inhibition reverses PABPN1-dependent muscle wasting
2022-02-28 | PXD011534 | Pride