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Identification of an outer membrane protease of Rickettsia rickettsii responsible for maturation of surface exposed autotransporters

ABSTRACT: Strains of R. rickettsii, the agent of Rocky Mountain spotted fever, differ greatly in the severity of the disease caused. The genetic differences responsible for this disparity are only now being uncovered. An avirulent, laboratory adapted strain of R. rickettsii fails to proteolytically process several large surface protein antigens. We have identified a protease that cleaves the protein precursors to their mature form. The gene encoding this protease is mutated in the avirulent strain. Complementation of the active form of the gene identifies proteolytic processing of surface antigens as important to virulence.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Rickettsia Rickettsii Str. 'sheila Smith' Rickettsia Rickettsii Str. Iowa

SUBMITTER: Yixiang Zhang

LAB HEAD: Ted Hackstadt

PROVIDER: PXD041355 | Pride | 2023-07-06

REPOSITORIES: Pride

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			Action	DRS
	20221027_1-percolator.xlsx	Xlsx
	20221027_1.raw	Raw
	20221027_2-percolator.xlsx	Xlsx
	20221027_2.raw	Raw
	20221027_3-percolator.xlsx	Xlsx

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