Dataset Information

A novel murine model of maternal micronutrient deficiencies and its implications in enteric inflammatory host-microbe interactions in the offspring

ABSTRACT: Micronutrient deficiency (MND i.e., lack of vitamins and minerals) during pregnancy is a major public health concern. Historically, studies have considered micronutrients in isolation; however, MNDs rarely occur alone. Furthermore, the impact of co-occurring MNDs on public health, mainly in shaping mucosal colonization by pathobionts from Enterobacteriaceae family, remain undetermined due to lack of relevant animal models.

INSTRUMENT(S): Bruker Daltonics instrument model

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Colon

SUBMITTER: Jenny Moon

LAB HEAD: B. Brett Finlay

PROVIDER: PXD041389 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant_1.6.17.0.zip	Other
	Ravi_01_C1_1_1531.d.1.dar	Other
	Ravi_02_C2_1_1532.d.1.dar	Other
	Ravi_03_C3_1_1533.d.1.dar	Other
	Ravi_04_C4_1_1534.d.1.dar	Other

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Publications

A Murine Model of Maternal Micronutrient Deficiencies and Gut Inflammatory Host-microbe Interactions in the Offspring.

Holani Ravi R Littlejohn Paula T PT Edwards Karlie K Petersen Charisse C Moon Kyung-Mee KM Stacey Richard G RG Bozorgmehr Tahereh T Gerbec Zachary J ZJ Serapio-Palacios Antonio A Krekhno Zakhar Z Donald Katherine K Foster Leonard J LJ Turvey Stuart E SE Finlay B Brett BB

Cellular and molecular gastroenterology and hepatology 20240201 5

<h4>Background & aims</h4>Micronutrient deficiency (MND) (ie, lack of vitamins and minerals) during pregnancy is a major public health concern. Historically, studies have considered micronutrients in isolation; however, MNDs rarely occur alone. The impact of co-occurring MNDs on public health, mainly in shaping mucosal colonization by pathobionts from the Enterobacteriaceae family, remains undetermined due to lack of relevant animal models.<h4>Methods</h4>To establish a maternal murine model of ...[more]

PMID: 38307490

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Project description:The effect of pre and periconceptional multiple micronutrient supplementation on methylation of CpG loci within CpG islands associated with 14,000 genes across the human genome has been investigated in the offspring of a cohort of Gambian women participating in a controlled double blinded trial. Methylation levels in placebo and micronutrient supplemented cohorts were compared in genomic DNA from cord blood (35 placebo and 21 micronutrient supplemented) and in circulating blood samples (14 placebo and 9 micronutrient supplemented) drawn from the same cohort at 9 months old. A small number of differentially methylated CpG loci were identified in cord blood (14 in males and 21 in females) and a larger number in the 9 month infant comparison (108 in males and 106 in females). Seven differentially methylated loci in males and 8 in females persisted from cord to infant. These findings indicate that micronutrient supplementation pre-conception or early in embryonic development is potentially associated with programming of gene activity at birth, which is maintained into early infanthood. Strikingly, the loci affected by micronutrient supplementation differed between males and females, with no shared changes in cord blood and only 5 shared changes at 9 months. Additionally, a large number of CpG loci showed variation in methylation level when comparing 9-month samples to cord blood samples. These postnatal changes were more consistent between sexes, with 85% of female alterations being found as a subset of male changes in the placebo cohort and 62% of the female changes shared with males in the supplemented cohort. Taken together, the results suggest that there is a core developmental program shared between the sexes that is unaffected by nutrient supplementation, but that there are also sex-specific developmental changes which are altered under conditions of micronutrient supplementation and deficiency. Bisulphite converted DNA from 59 cord (36 placebo and 23 treated) and 25 infant peripheral blood (15 placebo and 10 treated) samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2. (For further analysis, five were excluded during quality control leaving 56 cord (35 placebo and 21 treated) and 23 infant peripheral blood (14 placebo and 9 treated) samples. Full data set of 84 samples were submitted to GEO)