Dataset Information

Identification of DYRK3 interaction partners by immunoprecipitation-MS and TurboID

ABSTRACT: Proteins that interact with overexpressed GFP-DYRK3 were pulled down for identification by LC-MS/MS. Interaction strength and likelihood were calculated by comparison to pulldown of GFP alone. Data were compared to Turbo-ID proximity labelling data for DYRK3 in HeLa cells to identify consistently associated proteins. These included many secretory proteins. Further experiments established that DYRK3 phosphorylates SEC16A to regulate the liquid-liquid phase separation of ER exit sites.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Hela Cell

SUBMITTER: Katrina Meyer

LAB HEAD: Lucas Pelkmans

PROVIDER: PXD041550 | Pride | 2023-06-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200316_002_S214249_DYRK3_TurboID_2_cntrl1_Group_1_20200317175607.raw	Raw
	20200316_006_S214250_DYRK3_TurboID_2_cntrl2_Group_1_20200318032419.raw	Raw
	20200316_011_S214257_DYRK3_TurboID_2_5h_3_Group_3_20200318133435.raw	Raw
	20200316_012_S214256_DYRK3_TurboID_2_5h_2_Group_3_20200318155638.raw	Raw
	20200316_020_S214251_DYRK3_TurboID_2_cntrl3_Group_1.raw	Raw

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Identification of DYRK3 interaction partners by immunoprecipitation-MS and TurboID

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