Proteomics

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Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae Chlorella sorokiniana and Chlamydomonas reinhardtii


ABSTRACT: The accumulation of triacylglycerol (TAG) as a storage compound in eukaryotic algae has been the subject of extensive studies over the last 25 years. The model industrial alga Chlorella sorokiniana accumulates TAG and other storage compounds under nitrogen (N)-limited growth. Previously we used transcriptomics to explore the regulation of TAG synthesis in C. sorokiniana. Surprisingly, our analysis showed that the expression of several key genes encoding enzymes involved in plastidic fatty acid synthesis are significantly repressed. Metabolic labeling with radiolabeled acetate showed that de novo fatty acid synthesis is indeed downregulated under N-limitation. Likewise, inhibition of the Target of Rapamycin kinase (TOR), a key regulator of metabolism and growth, decreased fatty acid synthesis. We compared the changes induced in the quantitative proteome and phosphoproteome of cells under N-limitation or TOR inhibition and found extensive overlap between the N-limited and TOR-inhibited conditions. We also detected variations in the phosphorylation of TOR complex proteins under N-limitation, indicating that TOR signaling is altered. Our results indicate that under N-limitation there is significant metabolic remodeling, including fatty acid synthesis, mediated by TOR signaling. We find that TOR-mediated metabolic remodeling of fatty acid synthesis under N-limitation is conserved in the chlorophyte algae Chlorella sorokiniana and Chlamydomonas reinhardtii.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Chlorella Sorokiniana

TISSUE(S): Cell Culture

SUBMITTER: Sophe Alvarez  

LAB HEAD: Sophie Alvarez

PROVIDER: PXD041691 | Pride | 2024-10-17

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
20190927_JV_1x2_NA.raw Raw
20190927_JV_2x2_NB.raw Raw
20190927_JV_3x2_NC.raw Raw
20190927_JV_4x2_TA.raw Raw
20190927_JV_5x2_TB.raw Raw
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Publications

Nitrogen starvation leads to TOR kinase-mediated downregulation of fatty acid synthesis in the algae Chlorella sorokiniana and Chlamydomonas reinhardtii.

Vijayan Jithesh J   Alvarez Sophie S   Naldrett Michael J MJ   Morse Wyatt W   Maliva Amanda A   Wase Nishikant N   Riekhof Wayne R WR  

BMC plant biology 20240806 1


<h4>Background</h4>When subject to stress conditions such as nutrient limitation microalgae accumulate triacylglycerol (TAG). Fatty acid, a substrate for TAG synthesis is derived from de novo synthesis or by membrane remodeling. The model industrial alga Chlorellasorokiniana accumulates TAG and other storage compounds under nitrogen (N)-limited growth. Molecular mechanisms underlying these processes are still to be elucidated.<h4>Result</h4>Previously we used transcriptomics to explore the regul  ...[more]

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