Proteomics

Dataset Information

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Convergent origin and accelerated evolution of vesicle-associated RhoGAP proteins in two unrelated parasitoid wasps


ABSTRACT: Gene duplication, accompanied by modification of the expression and/or function of one of the duplicates under the action of positive selection, followed by further duplication to produce multigene families of toxins is a well-documented process in venomous animals. This evolutionary model has been less described in parasitoid wasps, which use maternal fluids, including venom, to protect their eggs from encapsulation by the host immune system. The leptopilina venom proteomic data were used to evidence that specific RhoGAPs formed a family of protein that are associated with vesicles that act as transport systems to deliver them in the immune cells of their drosophila larval host. We showed that the gene encoding the cellular RacGAP1 is at the origin of the virulent RhoGAP family formed by successive duplications that evolved under positive selection. Almost all of these RhoGAPs lost their GAP activity and GTPase binding ability due to mutations in key amino acids suggesting new function(s) and mechanism of action in host cells that remain to be elucidated.

INSTRUMENT(S): Q-Tof Ultima

ORGANISM(S): Leptopilina Boulardi Leptopilina Heterotoma

TISSUE(S): Venom Sac

SUBMITTER: Maya BELGHAZI  

LAB HEAD: Jean Luc gatti

PROVIDER: PXD041695 | Pride | 2023-06-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
F008108.dat Other
F008109.dat Other
F008110.dat Other
F008111.dat Other
F008112.dat Other
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Publications

Extensive inter- and intraspecific venom variation in closely related parasites targeting the same host: the case of Leptopilina parasitoids of Drosophila.

Colinet Dominique D   Deleury Emeline E   Anselme Caroline C   Cazes Dominique D   Poulain Julie J   Azema-Dossat Carole C   Belghazi Maya M   Gatti Jean-Luc JL   Poirié Marylène M  

Insect biochemistry and molecular biology 20130402 7


The arms race between immune suppressive parasites that produce virulence factors and hosts that evolve resistance to these factors is suggested to be a key driver for the diversification of both partners. However, little is known regarding the diversity of virulence factors in closely related parasites or the mechanisms underlying the variation of virulence. One of the best-described model to address this issue is the interaction between Leptopilina parasitic wasps and their Drosophila hosts, i  ...[more]

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