Dataset Information

Proteomic characterization of extracellular membrane vesicles from Streptococcus sanguinis

ABSTRACT: Streptococcus sanguinis, a Gram-positive oral commensal, is highly prevalent in the oral cavity and well-characterized for its ability to antagonize oral pathobionts. We have found that production and dissemination of membrane vesicles by S. sanguinis is dependent on environmental and community factors. Co-culture with interacting commensal Corynnyebacterium durum, as well as with the periodontal pathobiont Filifactor alocis had no effect on S. sanguinis vesicle number and size, whereas the periodontal pathobiont Porphyromonas gingivalis abolished S. sanguinis vesicle production. Using both correlation and differential expression analyses to examine the transcriptomic changes underlying vesicle production, we found that differential expression of genes encoding proteins related to the cytoplasmic membrane and peptidoglycan correlate with the abundance of membrane vesicles. Proteomic characterizations of the vesicle cargo identified a variety of proteins, including those predicted to influence host interactions or host immune responses. Cell culture studies of gingival epithelial cells demonstrated that both crude and highly purified membrane vesicles could induce the expression of IL-8, TNF-α, IL-1β, and Gro-α within 6 hours of inoculation at levels comparable to whole cells. OTaken together, our findings suggest that production of membrane vesicles by S. sanguinis is heavily influenced by community and environmental factors, and plays an important role in communication with host cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Streptococcus Sanguinis Sk36

SUBMITTER: Emily Helliwell

LAB HEAD: Jens Kreth

PROVIDER: PXD041791 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	111921_Helliwel_MemVes_1.raw	Raw
	111921_Helliwel_MemVes_2.raw	Raw
	111921_Helliwel_MemVes_3.raw	Raw
	111921_Helliwel_MemVes_4.raw	Raw
	111921_Helliwel_MemVes_S_sanguinis_1peptides.xlsx	Xlsx

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Publications

Environmental influences on Streptococcus sanguinis membrane vesicle biogenesis.

Helliwell Emily E Choi Dongseok D Merritt Justin J Kreth Jens J

The ISME journal 20230624 9

Membrane vesicles are produced by Gram-negative and Gram-positive bacteria. While membrane vesicles are potent elicitors of eukaryotic cells and involved in cell-cell communication, information is scarce about their general biology in the context of community members and the environment. Streptococcus sanguinis, a Gram-positive oral commensal, is prevalent in the oral cavity and well-characterized for its ability to antagonize oral pathobionts. We have found that production and dissemination of ...[more]

PMID: 37355741

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