Dataset Information

Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion and Migration in THP-1 Cells

ABSTRACT: The interaction between monocytes and endothelial cells in inflammation is central to chemoattraction, adhesion, and transendothelial migration. Key players such as selectins and their ligands, integrins and other adhesion molecules and their function in these processes are well studied. Toll-like receptor 2 (TLR2), expressed on monocytes, is critical for sensing invading pathogens and initiating a rapid and effective immune response. However, the extended role of TLR2 in monocyte adhesion and migration has only been partially elucidated. To address this question, we performed several functional cell-based assays using monocyte-like wild type (WT), TLR2-knock-out (KO) and, TLR2-knock-in (KI) THP-1 cells. We found that TLR2 promotes faster and stronger adhesion of monocytes to the endothelium and a more intense endothelial barrier disruption after endothelial activation. In addition, we performed quantitative mass-spectrometry, STRING protein analysis, and RT-qPCR, which revealed the association of TLR2 with specific integrins, but also uncovered novel proteins affected by TLR2. In conclusion, we could show that unstimulated TLR2 affects cell adhesion, endothelial barrier disruption, migration, and actin polymerization.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Monocyte, Cell Culture

SUBMITTER: Benjamin Neuditschko

LAB HEAD: Franz Herzog

PROVIDER: PXD041819 | Pride | 2023-10-24

REPOSITORIES: Pride

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Beyond Pattern Recognition: TLR2 Promotes Chemotaxis, Cell Adhesion, and Migration in THP-1 Cells.

Colleselli Katrin K Ebeyer-Masotta Marie M Neuditschko Benjamin B Stierschneider Anna A Pollhammer Christopher C Potocnjak Mia M Hundsberger Harald H Herzog Franz F Wiesner Christoph C

Cells 20230519 10

The interaction between monocytes and endothelial cells in inflammation is central to chemoattraction, adhesion, and transendothelial migration. Key players, such as selectins and their ligands, integrins, and other adhesion molecules, and their functions in these processes are well studied. Toll-like receptor 2 (TLR2), expressed in monocytes, is critical for sensing invading pathogens and initiating a rapid and effective immune response. However, the extended role of TLR2 in monocyte adhesion a ...[more]

PMID: 37408259

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			Action	DRS
	DIA_NN_GPF_report.tsv	Tabular
	DIA_NN_main_report.tsv	Tabular
	THP-1_KI_1.raw	Raw
	THP-1_KI_2.raw	Raw
	THP-1_KI_3.raw	Raw