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Dynamics of de novo heterochromatin assembly and disassembly at replication forks ensures fork stability

ABSTRACT: Analysis of histone PTM levels in proliferating, quiescent and HU-treated TIG3 fibroblasts. TIG3 fibroblasts were cultured in the absence or presence of HU (600 μM) for at least 6 days, rendering cells quiescent due to contact inhibition or long-term exposure to replication stress, respectively. The dataset contains RAW MS data used for histone PTM quantification presented in Gaggioli et al, 2023 (Fig.1c, Extended Data Fig. 1f and 2c)

INSTRUMENT(S): TSQ Vantage, Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Andrey Tvardovskiy

LAB HEAD: Nitika Taneja

PROVIDER: PXD041914 | Pride | 2023-05-11

REPOSITORIES: Pride

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Project description:Chromatin structure and function is maintained by dynamic protein-protein and protein-nucleic acid interactions. Histones are a family of proteins that are abundant chromatin constituents and that carry numerous post-translational modifications (PTMs). Histone PTMs mediate a variety of biological activities, including recruitment of enzymatic readers, writers and erasers that modulate protein activities, DNA replication, transcription and repair. Individual histone molecules contain multiple co-existing PTMs some of which exhibit crosstalk, i.e. coordinated or mutually exclusive activities. We here present an integrated experimental and computational approach for systems level molecular characterization of PTMs and PTM crosstalk. Using wildtype and engineered mouse embryonic stem cells with perturbations in the Polycomb Repressive Complex 1 (PRC1, suz12-/-), PRC2 (Ring1A/b-/-) and DNA methyltransferases (Dnmt1/3a/3b-/-) we performed comprehensive PTM analysis of histone H3 tails. We identified unique histone H3 PTM features of each of the four cell lines and we detected common combinatorial PTM features across cell lines. Using quantitative middle-down proteomics combined with probabilistic and statistical data analysis we extracted histone H3 PTM profiles for all four mESC systems. PTM crosstalk emerged as mutually exclusive histone PTMs or coordinately regulated PTMs independent of histone peptide abundance in the four model systems. We detected positive crosstalk between adjacent mono-methylated marks but strong negative crosstalk among most of the seven characterized di- and tri-methylations on lysines. We report novel features of PTM interplay involving hitherto poorly characterized arginine methylation and lysine methylation sites in histone H3, including H3R2me, H3R8me and H3K37me, which exhibited specific PTM codes suggesting a particular role in chromatin. All histone H3 PTM data is available in our publicly available CrossTalkDB repository at http://crosstalkdb.bmb.sdu.dk

			Action	DRS
	Day_0_rep_1.RAW	Raw
	Day_0_rep_2.RAW	Raw
	Day_0_rep_3.RAW	Raw
	Day_1_minus_HU_rep_1.RAW	Raw
	Day_1_minus_HU_rep_2.RAW	Raw

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Dynamics of de novo heterochromatin assembly and disassembly at replication forks ensures fork stability

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