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Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach

ABSTRACT: The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicated that HP1021 modulates H. pylori's response to oxidative stress. HP1021 controlled the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins were differently expressed in the HP1021 deletion mutant. HP1021 regulated typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. We identified HP1021 as the first molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes was relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controlled glucose consumption by directly regulating the gluP transporter and had an important impact on maintaining the energetic balance in the cell.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Helicobacter Pylori J99 (campylobacter Pylori J99)

SUBMITTER: Christina Ludwig

LAB HEAD: Christina Ludwig

PROVIDER: PXD041978 | Pride | 2023-09-20

REPOSITORIES: Pride

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			Action	DRS
	26695_HP_final.fasta	Fasta
	BBM_649_P108_21_03_VEB_001.raw	Raw
	BBM_649_P108_21_03_VEB_002.raw	Raw
	BBM_649_P108_21_03_VEB_003.raw	Raw
	BBM_649_P108_21_03_VEB_004.raw	Raw

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