Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PolQ peptide 4S-P (1482-P/86-P/88-P/93-P) binding partners identification

ABSTRACT: We characterized residus of PolQ which are phosphorylated during mitotis. We aimed to identify the partners of the phosphorylated residus of PolQ.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Vanessa Masson

LAB HEAD: Damarys Loew

PROVIDER: PXD042167 | Pride | 2023-09-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Design_PRIDE.xlsx	Xlsx
	E0980FD.msf	Msf
	E0980FD.raw	Raw
	E0981FD.msf	Msf
	E0981FD.raw	Raw

Items per page:

1 - 5 of 31

Publications

Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis.

Gelot Camille C Kovacs Marton Tibor MT Miron Simona S Mylne Emilie E Haan Alexis A Boeffard-Dosierre Liza L Ghouil Rania R Popova Tatiana T Dingli Florent F Loew Damarys D Guirouilh-Barbat Josée J Del Nery Elaine E Zinn-Justin Sophie S Ceccaldi Raphael R

Nature 20230906 7978

DNA double-strand breaks (DSBs) are deleterious lesions that challenge genome integrity. To mitigate this threat, human cells rely on the activity of multiple DNA repair machineries that are tightly regulated throughout the cell cycle1. In interphase, DSBs are mainly repaired by non-homologous end joining and homologous recombination2. However, these pathways are completely inhibited in mitosis3-5, leaving the fate of mitotic DSBs unknown. Here we show that DNA p ...[more]

PMID: 37674080

Similar Datasets

Extracellular vesicles from fibroblasts stimulated by FGF2

Project description:We compared proteomic analysis of extracellular vesicles (EVs) secreted from human dermal fibroblasts, either control or stimulated by FGF2 and after immunoprecipitation with FGF2-coated beads to isolated FGF2-positive EVs.

2022-12-20 | PXD033141 | Pride

Quantitative proteomic analysis of proteins bound to rs452384-T or rs452384-C

Project description:This project focuses on the functional characterization of a risk variant (SNP) implicated in the predisposition to uveal melanoma, rs452384. We sought to determine whether some nuclear factors could bind with allele-specificity a DNA sequence centered around rs452384 to regulate gene expression. We sought to identify by quantitative mass spectrometry the nuclear proteins bound to double-stranded DNA probes with identical DNA sequences except for rs452384-C or T alleles in the center of the probe. The goal was to quantify the binding of transcription factors to the DNA probes, and to select those enriched (or specific) to the C or T allele of rs452384. To identify proteins specifically bound to rs452384, we compared binding partners to those obtained with a negative control probe known not to recognize any transcription factor.

2022-11-15 | PXD028954 | Pride

DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of Lamin A/C

Project description:The integrity of the nuclear envelope (NE) is essential to maintain the structural stability of the nucleus. Rupture of the NE has been frequently observed in cancer cells, especially in the context of mechanical challenges, such as physical confinement and migration. However, spontaneous NE rupture events have also been described, without any obvious physical challenges to the cell. The molecular mechanism(s) of these spontaneous NE rupture events remain to be explored. Here, we show that DNA damage and subsequent ATR activation can lead to NE rupture. Upon DNA damage, Lamin A/C is phosphorylated in an ATR-dependent manner, leading to changes in lamina assembly and, ultimately, NE rupture. In addition, we show that cancer cells with intrinsic DNA repair defects undergo frequent events of DNA damage-induced NE rupture, which renders them extremely sensitive to further NE perturbations. Exploiting this NE vulnerability could provide a new angle to complement traditional, DNA damage-based chemotherapy.

2023-10-09 | PXD040857 | Pride

Mouse - Identification of CTNND2 partners in synaptic fractions of P15 mouse brains

Project description:This project aims to identify CTNND2 partners in developing synapses

2024-10-03 | PXD036487 | Pride

Mouse - SRGAP2 partners in synaptic fractions of P15 mouse brains

Project description:This projects aims to identify the proteins that are in complex with SRGAP2 in developing synapses

2024-10-03 | PXD036488 | Pride

RagG interactome analysis in human breast MDA-MB-231 cells

Project description:RagC was immunoprecipitated from human breast cancer MDA-MB-231 cells treated or not with the Arp2/3 complex inhibitor, CK666, and the RagC interactome was analyzed by mass-spectrometry.

2023-09-04 | PXD027100 | Pride

Translation regulation mechanisms in astrocytes

Project description:Identifying the proteins associated with the polyribosomes in astrocytes from the mouse brain to unveil translation regulation mechanisms in these cells.

2023-07-20 | PXD033121 | Pride

Discovery of RUF6 ncRNA interacting proteins involved in P. falciparum immune evasion

Project description:RUF6 is a ncRNA gene family that is transcribed by RNA Polymerase III but actively regulates the Pol II-transcribed var virulence gene family. Understanding how RUF6 ncRNA connect to downstream effectors is lacking. We developed an RNA-directed proteomic discovery (ChIRP-MS) protocol to identify in vivo RUF6 ncRNA protein interactions. The RUF6 ncRNA interactome was purified with biotinylated antisense oligonucleotides. Quantitative label-free mass spectrometry identified several unique proteins linked to gene transcription including. Affinity purification of Pf-DDX5 identified proteins originally found by our RUF6-ChIRP protocol, validating the technique’s robustness for identifying ncRNA interactomes in P. falciparum. Our work identifies a RUF6 ncRNA protein complex that interacts with RNA Pol II to sustain var gene expression.

2022-11-15 | PXD036801 | Pride

GFP-CYYR1 interactome vs. GFP in HEK293 cells

Project description:Cell lysates from HEK293 cells individually transfected with GFP or GFP-CYYR1 were purified on GFP-Trap affinity resin and analyzed by quantitative label-free mass spectrometry. Experiments were performed in 5 biological replicates for each condition.

2024-07-25 | PXD050895 | Pride

Environmental inhibition of RNA Pol III reduces malaria parasite virulence (during asymptomatic infection)

Project description:During the dry season, in many African regions, persistent asymptomatic malaria cases have been linked to downregulated virulence gene expression encoded by the var gene family, resulting in low adhesion and increased circulation time of Plasmodium falciparum-infected erythrocytes 1. Several studies have investigated associations between parasite transcription and clinical severity, but the underlying process that results in lower virulence gene expression in asymptomatic malaria remains unknown. Here we report an RNA Polymerase III-dependent mechanism that in response to external stimuli, decreases var gene transcription and parasite multiplication rate. The singular expression of var gene members, while under tight epigenetic regulation by the parasite 2, is additionally controlled by a family of ncRNA, termed RUF6 3. We show that Pol III transcription of tRNAs and RUF6 is severely decreased in field isolates of asymptomatic individuals infected with P. falciparum during the dry season. We identified Maf1 as a negative effector of RUF6 ncRNA transcription and show that external stimuli depend on plasmodial Maf1 to trigger lower expression of RNA Pol III-transcribed genes. Our results uncover a TOR-independent unprecedented signaling pathway in a protozoan pathogen that is highly relevant for establishing asymptomatic subclinical parasite persistence in the dry season.

2024-06-25 | PXD040576 | Pride