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Proteome-scale recombinant standard datasets for XL-MS

ABSTRACT: This submission includes the complete set of raw data generated from the analytical standard, the concept of which is described in our manuscript "Proteome-Scale Recombinant Standards And A Robust High-Speed Search Engine To Advance Cross-Linking MS-Based Interactomics". In this study, we develop a strategy to generate a well-controlled XL-MS standard by systematically mixing and cross-linking recombinant proteins. The standard can be split into independent datasets, each of which has the MS2-level complexity of a typical proteome-wide XL-MS experiment. This submission includes 5 raw datasets (batches 1-5), each comprising 64 randomly selected human proteins engaging in up to 224 allowed protein-protein interactions per dataset. The data were searched with Scout (v. 1.4.14, https://github.com/diogobor/Scout) and results are reported in mzIdentML 1.2.0 format. For the specific results reported in our original publication, please refer to PXD042173.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Diogo Borges Lima

LAB HEAD: Fan Liu

PROVIDER: PXD042173 | Pride | 2024-08-06

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	L1_20210727_MxR_FDR_firstbatch_DSSOplate2_B12.raw	Raw
	L1_20210727_MxR_FDR_firstbatch_DSSOplate2_E12.raw	Raw
	L1_20210727_MxR_FDR_firstbatch_DSSOplate2_H12.raw	Raw
	L1_20210806_MxR_FDR_firstbatch_DSSOplate_C10.raw	Raw
	L1_20210806_MxR_FDR_firstbatch_DSSOplate_C11.raw	Raw

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