Dataset Information

Multi-omics analysis of mutagenized S. rimosus reveals synergistic changes that drive oxytetracycline production towards industrial efficiency and opens a new route to heterologous polyketides

ABSTRACT: The present work provides a multi-omics systems-wide view on S. rimosus. Using genomics, transcriptomics, proteomics, and metabolomics, we compared the wild type with an OTC-overproducing derivative, previously obtained by classical mutagenesis. The integration of the data provided a deep insight into the underlying metabolic and regulatory networks that mediate high-level OTC formation. Strikingly, the overproducer revealed a synergistically activated supply of acetyl-CoA and malonyl CoA and increased abundance of various CoA thioesters.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Streptomyces Rimosus Subsp. Rimosus

SUBMITTER: Christian Rückert-Reed

LAB HEAD: Jörn Kalinowski

PROVIDER: PXD042182 | Pride | 2024-01-26

REPOSITORIES: Pride

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Systems biology of industrial oxytetracycline production in Streptomyces rimosus: the secrets of a mutagenized hyperproducer.

Beganovic Selma S Rückert-Reed Christian C Sucipto Hilda H Shu Wei W Gläser Lars L Patschkowski Thomas T Struck Ben B Kalinowski Jörn J Luzhetskyy Andriy A Wittmann Christoph C

Microbial cell factories 20231028 1

<h4>Background</h4>Oxytetracycline which is derived from Streptomyces rimosus, inhibits a wide range of bacteria and is industrially important. The underlying biosynthetic processes are complex and hinder rational engineering, so industrial manufacturing currently relies on classical mutants for production. While the biochemistry underlying oxytetracycline synthesis is known to involve polyketide synthase, hyperproducing strains of S. rimosus have not been extensively studied, limiting our knowl ...[more]

PMID: 37898787

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